验证数据展示
经过测试的应用
Positive WB detected in | Jurkat cells, A431 cells, EC109 cells, MOLT-4 cells, NK-92 cells |
Positive IHC detected in | human cervical cancer tissue, mouse brain tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Positive IF/ICC detected in | SH-SY5Y cells |
推荐稀释比
应用 | 推荐稀释比 |
---|---|
Western Blot (WB) | WB : 1:5000-1:50000 |
Immunohistochemistry (IHC) | IHC : 1:250-1:1000 |
Immunofluorescence (IF)/ICC | IF/ICC : 1:400-1:1600 |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
产品信息
68247-1-Ig targets KLK8 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse samples.
经测试应用 | WB, IHC, IF/ICC, ELISA Application Description |
经测试反应性 | human, mouse |
免疫原 | KLK8 fusion protein Ag5642 种属同源性预测 |
宿主/亚型 | Mouse / IgG2a |
抗体类别 | Monoclonal |
产品类型 | Antibody |
全称 | kallikrein-related peptidase 8 |
别名 | Kallikrein 8, Kallikrein-8, Kallikrein8, kallikrein related peptidase 8, EC:3.4.21.118 |
计算分子量 | 28 kDa |
观测分子量 | 28 kDa |
GenBank蛋白编号 | BC040887 |
基因名称 | KLK8 |
Gene ID (NCBI) | 11202 |
RRID | AB_2935333 |
偶联类型 | Unconjugated |
形式 | Liquid |
纯化方式 | Protein A purification |
UNIPROT ID | O60259 |
储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
KLK8(Kallikrein-8) is also named as NRPN, PRSS19, TADG14.It is a new member of the human kallikrein family, which is predicted to be secreted and expected to be present in biological fluids (PMID:12507964).The protein is detectable in ovarian cancer tissue extracts, serum, and ascites fluid, indicating that it may serve as a new ovarian cancer marker (PMID:12782581). KLK8 has one predicted glycosylation site (PMID:20940292) and it has some isoforms produced by alternative splicing.
实验方案
Product Specific Protocols | |
---|---|
WB protocol for KLK8 antibody 68247-1-Ig | Download protocol |
IHC protocol for KLK8 antibody 68247-1-Ig | Download protocol |
IF protocol for KLK8 antibody 68247-1-Ig | Download protocol |
Standard Protocols | |
---|---|
Click here to view our Standard Protocols |