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KLK8 Monoclonal antibody

KLK8 Monoclonal Antibody for WB, IF/ICC, ELISA

Host / Isotype

Mouse / IgG2a

Reactivity

Human

Applications

WB, IF/ICC, ELISA

Conjugate

Unconjugated

CloneNo.

2F3A8

Cat no : 68247-1-Ig

Print datasheet

Synonyms

hK8, HNP, Kallikrein 8, kallikrein related peptidase 8, KLK8, Neuropsin, NP, NRPN, Ovasin, PRSS19, Serine protease 19, Serine protease TADG 14, TADG14

验证数据展示

  • WB analysis using 68247-1-Ig

    WB analysis using 68247-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 68247-1-Ig (KLK8 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • WB analysis of EC109 using 68247-1-Ig

    WB analysis of EC109 using 68247-1-Ig

    EC109 cells were subjected to SDS PAGE followed by western blot with 68247-1-Ig (KLK8 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • WB analysis of A431 using 68247-1-Ig

    WB analysis of A431 using 68247-1-Ig

    A431 cells were subjected to SDS PAGE followed by western blot with 68247-1-Ig (KLK8 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • IF Staining of SH-SY5Y using 68247-1-Ig

    IF Staining of SH-SY5Y using 68247-1-Ig

    Immunofluorescent analysis of (4% PFA) fixed SH-SY5Y cells using KLK8 antibody (68247-1-Ig, Clone: 2F3A8 ) at dilution of 1:800 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L).

  • IF Staining of SH-SY5Y using 68247-1-Ig

    IF Staining of SH-SY5Y using 68247-1-Ig

    Immunofluorescent analysis of (4% PFA) fixed SH-SY5Y cells using KLK8 antibody (68247-1-Ig, Clone: 2F3A8 ) at dilution of 1:800 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L).

经过测试的应用

Positive WB detected inJurkat cells, A431 cells, EC109 cells, MOLT-4 cells, NK-92 cells
Positive IF/ICC detected inSH-SY5Y cells
Planning an IHC experiment? We recommend our IHCeasy KLK8 Ready-To-Use IHC Kit. KLK8 primary antibody included.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunofluorescence (IF)/ICCIF/ICC : 1:400-1:1600
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

68247-1-Ig targets KLK8 in WB, IF/ICC, ELISA applications and shows reactivity with Human samples.

Tested Applications WB, IF/ICC, ELISA Application Description
Tested Reactivity Human
Immunogen KLK8 fusion protein Ag5642 种属同源性预测
Host / Isotype Mouse / IgG2a
Class Monoclonal
Type Antibody
Full Name kallikrein-related peptidase 8
Synonyms hK8, HNP, Kallikrein 8, kallikrein related peptidase 8, KLK8, Neuropsin, NP, NRPN, Ovasin, PRSS19, Serine protease 19, Serine protease TADG 14, TADG14
Calculated Molecular Weight 28 kDa
Observed Molecular Weight28 kDa
GenBank Accession NumberBC040887
Gene Symbol KLK8
Gene ID (NCBI) 11202
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDO60259
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

KLK8(Kallikrein-8) is also named as NRPN, PRSS19, TADG14.It is a new member of the human kallikrein family, which is predicted to be secreted and expected to be present in biological fluids (PMID:12507964).The protein is detectable in ovarian cancer tissue extracts, serum, and ascites fluid, indicating that it may serve as a new ovarian cancer marker (PMID:12782581). KLK8 has one predicted glycosylation site (PMID:20940292) and it has some isoforms produced by alternative splicing.

实验方案

Product Specific Protocols
WB protocol for KLK8 antibody 68247-1-IgDownload protocol
IF protocol for KLK8 antibody 68247-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols