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验证数据展示

  • WB analysis using 68247-1-Ig (same clone as 68247-1-PBS)

    WB analysis using 68247-1-Ig (same clone as 68247-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 68247-1-Ig (KLK8 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 68247-1-PBS in a different storage buffer formulation.

  • WB analysis of EC109 using 68247-1-Ig (same clone as 68247-1-PBS)

    WB analysis of EC109 using 68247-1-Ig (same clone as 68247-1-PBS)

    EC109 cells were subjected to SDS PAGE followed by western blot with 68247-1-Ig (KLK8 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 68247-1-PBS in a different storage buffer formulation.

  • WB analysis of A431 using 68247-1-Ig (same clone as 68247-1-PBS)

    WB analysis of A431 using 68247-1-Ig (same clone as 68247-1-PBS)

    A431 cells were subjected to SDS PAGE followed by western blot with 68247-1-Ig (KLK8 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 68247-1-PBS in a different storage buffer formulation.

  • IHC staining of human cervical cancer using 68247-1-Ig (same clone as 68247-1-PBS)

    IHC staining of human cervical cancer using 68247-1-Ig (same clone as 68247-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue slide using 68247-1-Ig (KLK8 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 68247-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with KLK8 antibody included
  • IHC staining of human cervical cancer using 68247-1-Ig (same clone as 68247-1-PBS)

    IHC staining of human cervical cancer using 68247-1-Ig (same clone as 68247-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue slide using 68247-1-Ig (KLK8 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 68247-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with KLK8 antibody included
  • IHC staining of mouse brain using 68247-1-Ig (same clone as 68247-1-PBS)

    IHC staining of mouse brain using 68247-1-Ig (same clone as 68247-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 68247-1-Ig (KLK8 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 68247-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with KLK8 antibody included
  • IHC staining of mouse brain using 68247-1-Ig (same clone as 68247-1-PBS)

    IHC staining of mouse brain using 68247-1-Ig (same clone as 68247-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 68247-1-Ig (KLK8 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 68247-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with KLK8 antibody included
  • IF Staining of SH-SY5Y using 68247-1-Ig (same clone as 68247-1-PBS)

    IF Staining of SH-SY5Y using 68247-1-Ig (same clone as 68247-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed SH-SY5Y cells using KLK8 antibody (68247-1-Ig, Clone: 2F3A8 ) at dilution of 1:800 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 68247-1-PBS in a different storage buffer formulation.

  • IF Staining of SH-SY5Y using 68247-1-Ig (same clone as 68247-1-PBS)

    IF Staining of SH-SY5Y using 68247-1-Ig (same clone as 68247-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed SH-SY5Y cells using KLK8 antibody (68247-1-Ig, Clone: 2F3A8 ) at dilution of 1:800 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 68247-1-PBS in a different storage buffer formulation.

KLK8 Monoclonal antibody, PBS Only

KLK8 Monoclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA
Cat No. 68247-1-PBS

产品说明书

CloneNo. 2F3A8

宿主/亚型

Mouse / IgG2a

种属反应性

human, mouse

应用

WB, IHC, IF/ICC, Indirect ELISA

别名

Kallikrein 8, Kallikrein-8, Kallikrein8, kallikrein related peptidase 8, EC:3.4.21.118

缓冲液配方:  PBS Only
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格: 

-/ -

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Planning an IHC experiment? We recommend our IHCeasy KLK8 Ready-To-Use IHC Kit. KLK8 primary antibody included.

产品信息

68247-1-PBS targets KLK8 in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with human, mouse samples.

经测试应用 WB, IHC, IF/ICC, Indirect ELISA Application Description
经测试反应性 human, mouse
免疫原 KLK8 fusion protein Ag5642 种属同源性预测
宿主/亚型 Mouse / IgG2a
抗体类别 Monoclonal
产品类型 Antibody
全称 kallikrein-related peptidase 8
别名 Kallikrein 8, Kallikrein-8, Kallikrein8, kallikrein related peptidase 8, EC:3.4.21.118
计算分子量 28 kDa
观测分子量28 kDa
GenBank蛋白编号BC040887
基因名称 KLK8
Gene ID (NCBI) 11202
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purification
UNIPROT IDO60259
储存缓冲液 PBS only , pH 7.3
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

KLK8(Kallikrein-8) is also named as NRPN, PRSS19, TADG14.It is a new member of the human kallikrein family, which is predicted to be secreted and expected to be present in biological fluids (PMID:12507964).The protein is detectable in ovarian cancer tissue extracts, serum, and ascites fluid, indicating that it may serve as a new ovarian cancer marker (PMID:12782581). KLK8 has one predicted glycosylation site (PMID:20940292) and it has some isoforms produced by alternative splicing.