• All
  • Primary Antibodies
  • Conjugated Antibodies for IF
  • Conjugated Antibodies for FC
  • Secondary Antibodies
  • Antibody Labeling KitsNew
  • ELISA Kits
  • IHC Kits
  • Magnetic Cell Separation Kits
  • Cytokines & Growth Factors
  • Neutralizing/activating Antibodies
  • Nanobody-based Reagents
  • Accessory Products and Kits
  • Fusion Proteins
×
×
Host
0
Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
0
Compatible Species
0
Conjugate
0

验证数据展示

  • WB analysis of human heart using 66123-1-Ig

    WB analysis of human heart using 66123-1-Ig

    human heart tissue were subjected to SDS PAGE followed by western blot with 66123-1-Ig (Kininogen 1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • IHC staining of human breast cancer using 66123-1-Ig

    IHC staining of human breast cancer using 66123-1-Ig

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 66123-1-Ig (Kininogen 1 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of human breast cancer using 66123-1-Ig

    IHC staining of human breast cancer using 66123-1-Ig

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 66123-1-Ig (Kininogen 1 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IF Staining of HeLa using 66123-1-Ig

    IF Staining of HeLa using 66123-1-Ig

    Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using Kininogen 1 antibody (66123-1-Ig, Clone: 10E8D11 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L).

Kininogen 1 Monoclonal antibody

Kininogen 1 Monoclonal Antibody for WB, IHC, IF/ICC, ELISA
Cat No. 66123-1-Ig

产品说明书

CloneNo. 10E8D11

宿主/亚型

Mouse / IgG1

种属反应性

human

应用

WB, IHC, IF/ICC, ELISA

别名

BDK, Fitzgerald factor, HMWK, Ile Ser Bradykinin Bradykinin, Kallidin I Lysyl bradykinin, kininogen 1, KNG, KNG1

缓冲液配方:  PBS and Azide
PBS and Azide
PBS Only
偶联物:  Unconjugated
Unconjugated
CoraLite® Plus 488
规格: 

-/ -

点击咨询售前客服

点击咨询售后客服

经过测试的应用

Positive WB detected inhuman heart tissue, human placenta tissue, human plasma tissue
Positive IHC detected inhuman breast cancer tissue, human ovary tumor tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHeLa cells
Planning an IF experiment? We recommend our CoraLite® Plus 488 conjugated versions of this antibody.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:5000-1:20000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

66123-1-Ig targets Kininogen 1 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human samples.

经测试应用 WB, IHC, IF/ICC, ELISA Application Description
经测试反应性 human
免疫原 Kininogen 1 fusion protein Ag19233 种属同源性预测
宿主/亚型 Mouse / IgG1
抗体类别 Monoclonal
产品类型 Antibody
全称 kininogen 1
别名 BDK, Fitzgerald factor, HMWK, Ile Ser Bradykinin Bradykinin, Kallidin I Lysyl bradykinin, kininogen 1, KNG, KNG1
计算分子量 644 aa, 72 kDa
观测分子量 68 kDa
GenBank蛋白编号BC060039
基因名称 Kininogen 1
Gene ID (NCBI) 3827
RRIDAB_2881522
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein G purification
UNIPROT IDP01042
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Kininogens are inhibitors of thiol proteases. Kininogen 1 plays important role in Kinin-kallikrein system. This gene is translated into High-molecular weight kininogen (HMWK) and low-molecular weight kininogen (LMWK) after alternative splicing. HMWK is produced by the liver together with prekallikrein. It acts mainly as a cofactor on coagulation and inflammation, and has no intrinsic catalytic activity. LMWK is produced locally by numerous tissues, and secreted together with tissue kallikrein.

实验方案

Product Specific Protocols
WB protocol for Kininogen 1 antibody 66123-1-IgDownload protocol
IHC protocol for Kininogen 1 antibody 66123-1-IgDownload protocol
IF protocol for Kininogen 1 antibody 66123-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols