• All
  • Primary Antibodies
  • Conjugated Antibodies for IF
  • Conjugated Antibodies for FC
  • Secondary Antibodies
  • Antibody Labeling KitsNew
  • ELISA Kits
  • IHC Kits
  • Magnetic Cell Separation Kits
  • Cytokines & Growth Factors
  • Neutralizing/activating Antibodies
  • Nanobody-based Reagents
  • Accessory Products and Kits
  • Fusion Proteins
×
×
Host
0
Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
0
Compatible Species
0
Conjugate
0

Kininogen 1 Monoclonal antibody, PBS Only

Kininogen 1 Monoclonal Antibody for WB,Indirect ELISA,IHC,IF

Host / Isotype

Mouse / IgG1

Reactivity

human

Applications

WB,Indirect ELISA,IHC,IF

Conjugate

Unconjugated

CloneNo.

10E8D11

Cat No : 66123-1-PBS

Print datasheet

Synonyms

BDK, Fitzgerald factor, HMWK, Ile Ser Bradykinin Bradykinin, Kallidin I Lysyl bradykinin, kininogen 1, KNG, KNG1

验证数据展示

  • WB analysis of human heart using 66123-1-Ig (same clone as 66123-1-PBS)

    WB analysis of human heart using 66123-1-Ig (same clone as 66123-1-PBS)

    human heart tissue were subjected to SDS PAGE followed by western blot with 66123-1-Ig (Kininogen 1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66123-1-PBS in a different storage buffer formulation.

  • IHC staining of human breast cancer using 66123-1-Ig (same clone as 66123-1-PBS)

    IHC staining of human breast cancer using 66123-1-Ig (same clone as 66123-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 66123-1-Ig (Kininogen 1 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66123-1-PBS in a different storage buffer formulation.

  • IHC staining of human breast cancer using 66123-1-Ig (same clone as 66123-1-PBS)

    IHC staining of human breast cancer using 66123-1-Ig (same clone as 66123-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 66123-1-Ig (Kininogen 1 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66123-1-PBS in a different storage buffer formulation.

  • IF Staining of HeLa using 66123-1-Ig (same clone as 66123-1-PBS)

    IF Staining of HeLa using 66123-1-Ig (same clone as 66123-1-PBS)

    Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using Kininogen 1 antibody (66123-1-Ig, Clone: 10E8D11 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 66123-1-PBS in a different storage buffer formulation.

产品信息

66123-1-PBS targets Kininogen 1 in WB, IF, IHC, Indirect ELISA applications and shows reactivity with human samples.

Tested Applications WB,Indirect ELISA,IHC,IF Application Description
Tested Reactivity human
Immunogen Kininogen 1 fusion protein Ag19233 种属同源性预测
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Full Name kininogen 1
Synonyms BDK, Fitzgerald factor, HMWK, Ile Ser Bradykinin Bradykinin, Kallidin I Lysyl bradykinin, kininogen 1, KNG, KNG1
Calculated Molecular Weight 644 aa, 72 kDa
Observed Molecular Weight 68 kDa
GenBank Accession NumberBC060039
Gene Symbol KNG1
Gene ID (NCBI) 3827
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
UNIPROT IDP01042
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

背景介绍

Kininogens are inhibitors of thiol proteases. Kininogen 1 plays important role in Kinin-kallikrein system. This gene is translated into High-molecular weight kininogen (HMWK) and low-molecular weight kininogen (LMWK) after alternative splicing. HMWK is produced by the liver together with prekallikrein. It acts mainly as a cofactor on coagulation and inflammation, and has no intrinsic catalytic activity. LMWK is produced locally by numerous tissues, and secreted together with tissue kallikrein.