• All
  • Primary Antibodies
  • Conjugated Antibodies for IF
  • Conjugated Antibodies for FC
  • Secondary Antibodies
  • Antibody Labeling KitsNew
  • ELISA Kits
  • IHC Kits
  • Magnetic Cell Separation Kits
  • Cytokines & Growth Factors
  • Neutralizing/activating Antibodies
  • Nanobody-based Reagents
  • Accessory Products and Kits
  • Fusion Proteins
×
×
Host
0
Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
0
Compatible Species
0
Conjugate
0

验证数据展示

  • WB analysis using 66870-1-Ig

    WB analysis using 66870-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 66870-1-Ig (KPNA2 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

  • IHC staining of human skin cancer using 66870-1-Ig

    IHC staining of human skin cancer using 66870-1-Ig

    Immunohistochemical analysis of paraffin-embedded skin cancer slide using 66870-1-Ig (KPNA2 antibody) at dilution of 1:600 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with KPNA2 antibody included
  • IF Staining of MCF-7 using 66870-1-Ig

    IF Staining of MCF-7 using 66870-1-Ig

    Immunofluorescent analysis of (4% PFA) fixed MCF-7 cells using KPNA2 antibody (66870-1-Ig, Clone: 2G9C1 ) at dilution of 1:800 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

KPNA2 Monoclonal antibody

KPNA2 Monoclonal Antibody for WB, IHC, IF/ICC, ELISA
Cat No. 66870-1-Ig

产品说明书

CloneNo. 2G9C1

宿主/亚型

Mouse / IgG1

种属反应性

human

应用

WB, IHC, IF/ICC, ELISA

别名

Karyopherin subunit alpha-2, IPOA1, IPOA 1, IPO A1, Importin subunit alpha-1

缓冲液配方:  PBS and Azide
PBS and Azide
PBS Only
偶联物:  Unconjugated
Unconjugated
CoraLite® Plus 488
规格: 

-/ -

点击咨询售前客服

点击咨询售后客服

经过测试的应用

Positive WB detected inHeLa cells, HEK-293 cells, MCF-7 cells, Jurkat cells, Raji cells
Positive IHC detected inhuman skin cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inMCF-7 cells
Planning an IHC experiment? We recommend our IHCeasy KPNA2 Ready-To-Use IHC Kit. KPNA2 primary antibody included.
Planning an IF experiment? We recommend our CoraLite® Plus 488 conjugated versions of this antibody.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:1000-1:5000
Immunohistochemistry (IHC)IHC : 1:300-1:1200
Immunofluorescence (IF)/ICCIF/ICC : 1:400-1:1600
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

66870-1-Ig targets KPNA2 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human samples.

经测试应用 WB, IHC, IF/ICC, ELISA Application Description
经测试反应性 human
免疫原 KPNA2 fusion protein Ag28386 种属同源性预测
宿主/亚型 Mouse / IgG1
抗体类别 Monoclonal
产品类型 Antibody
全称 karyopherin alpha 2 (RAG cohort 1, importin alpha 1)
别名 Karyopherin subunit alpha-2, IPOA1, IPOA 1, IPO A1, Importin subunit alpha-1
计算分子量 57.8 kDa
观测分子量 55-58 kDa
GenBank蛋白编号BC005978
基因名称 KPNA2
Gene ID (NCBI) 3838
RRIDAB_2882207
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purification
UNIPROT IDP52292
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

KPNA2 (karyopherin α2) is a nuclear import factor involved in the nucleocytoplasmic transport system. It is functionally involved in the tumor progression and is elevated in multiple cancers. KPNA2 expression may be a useful prognostic biomarker to monitor cancer prognosis.

实验方案

Product Specific Protocols
WB protocol for KPNA2 antibody 66870-1-IgDownload protocol
IHC protocol for KPNA2 antibody 66870-1-IgDownload protocol
IF protocol for KPNA2 antibody 66870-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols