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KPNA2 Monoclonal antibody

KPNA2 Monoclonal Antibody for IF/ICC, WB, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human

Applications

IF/ICC, WB, ELISA

Conjugate

Unconjugated

CloneNo.

2G9C1

Cat No : 66870-1-Ig

Print datasheet

Synonyms

Importin subunit alpha 2, IPOA1, Karyopherin subunit alpha 2, KPNA2, QIP2, RAG cohort protein 1, RCH1, SRP1, SRP1 alpha, SRP1alpha

验证数据展示

  • WB analysis using 66870-1-Ig

    WB analysis using 66870-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 66870-1-Ig (KPNA2 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

  • IF Staining of MCF-7 using 66870-1-Ig

    IF Staining of MCF-7 using 66870-1-Ig

    Immunofluorescent analysis of (4% PFA) fixed MCF-7 cells using KPNA2 antibody (66870-1-Ig, Clone: 2G9C1 ) at dilution of 1:800 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

经过测试的应用

Positive WB detected inHeLa cells, HEK-293 cells, MCF-7 cells, Jurkat cells, Raji cells
Positive IF detected inMCF-7 cells
Planning an IHC experiment? We recommend our IHCeasy KPNA2 Ready-To-Use IHC Kit. KPNA2 primary antibody included.
Planning an IF experiment? We recommend our CoraLite® Plus 488 conjugated versions of this antibody.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:5000
Immunofluorescence (IF)IF : 1:400-1:1600
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

66870-1-Ig targets KPNA2 in WB, IF, ELISA applications and shows reactivity with Human samples.

Tested Applications IF/ICC, WB, ELISA Application Description
Tested Reactivity Human
Immunogen KPNA2 fusion protein Ag28386 种属同源性预测
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Full Name karyopherin alpha 2 (RAG cohort 1, importin alpha 1)
Synonyms Importin subunit alpha 2, IPOA1, Karyopherin subunit alpha 2, KPNA2, QIP2, RAG cohort protein 1, RCH1, SRP1, SRP1 alpha, SRP1alpha
Calculated Molecular Weight 57.8 kDa
Observed Molecular Weight 55-58 kDa
GenBank Accession NumberBC005978
Gene Symbol KPNA2
Gene ID (NCBI) 3838
RRIDAB_2882207
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
UNIPROT IDP52292
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

KPNA2 (karyopherin α2) is a nuclear import factor involved in the nucleocytoplasmic transport system. It is functionally involved in the tumor progression and is elevated in multiple cancers. KPNA2 expression may be a useful prognostic biomarker to monitor cancer prognosis.

实验方案

Product Specific Protocols
WB protocol for KPNA2 antibody 66870-1-IgDownload protocol
IF protocol for KPNA2 antibody 66870-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols