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Importin Beta Polyclonal antibody

Importin Beta Polyclonal Antibody for WB, IHC, IF/ICC, FC (Intra), ELISA
Cat No. 10077-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IHC, IF/ICC, FC (Intra), ELISA and More (3)

KPNB1, Importin-90, Importin subunit beta-1, Importin subunit beta 1, Importin 90

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
CoraLite® Plus 488
规格: 

-/ -


经过测试的应用

Positive WB detected inA2780 cells, mouse brain tissue, rat brain tissue, HeLa cells, NIH/3T3 cells, C6 cells, HT-1080 cells, PC-12 cells
Positive IHC detected inhuman colon cancer tissue, mouse brain tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHeLa cells
Positive FC (Intra) detected inJurkat cells
Planning an IHC experiment? We recommend our IHCeasy KPNB1 Ready-To-Use IHC Kit. KPNB1 primary antibody included.
Planning an IF experiment? We recommend our CoraLite® Plus 488 conjugated versions of this antibody.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:1000-1:4000
Immunohistochemistry (IHC)IHC : 1:20-1:200
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

10077-1-AP targets Importin Beta in WB, IHC, IF/ICC, FC (Intra), IP, CoIP, ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, IF/ICC, FC (Intra), ELISA Application Description
文献引用应用WB, IHC, IF, IP, CoIP
经测试反应性 human, mouse, rat
文献引用反应性human, mouse
免疫原 Importin Beta fusion protein Ag0122 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 karyopherin (importin) beta 1
别名 KPNB1, Importin-90, Importin subunit beta-1, Importin subunit beta 1, Importin 90
计算分子量 97 kDa
观测分子量 90-97 kDa
GenBank蛋白编号BC003572
基因名称 KPNB1
Gene ID (NCBI) 3837
RRIDAB_2133977
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ14974
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Importin beta1, also known karyopherin beta or KPNB1, is a nuclear transport receptor that plays a key role in the nuclear import process. Importin beta1 and importin alpha work in concert to transport cargo into the nucleus, functioning as an essential component of the classical nuclear localization signal (NLS) pathway. Elevated expression of importin beta1 has been found in cervical tumors. (22125623)

实验方案

Product Specific Protocols
WB protocol for Importin Beta antibody 10077-1-APDownload protocol
IHC protocol for Importin Beta antibody 10077-1-APDownload protocol
IF protocol for Importin Beta antibody 10077-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB,IP

Cell Res

Neddylation of PTEN regulates its nuclear import and promotes tumor development.

Authors - Ping Xie
WB

Cell Rep

CGG repeats in the human FMR1 gene regulate mRNA localization and cellular stress in developing neurons

Authors - Carissa L Sirois
humanWB

Oncogene

KPNB1 inhibition disrupts proteostasis and triggers unfolded protein response-mediated apoptosis in glioblastoma cells.

Authors - Zhi-Chuan Zhu
  • KD Validated
humanWB,IP

J Transl Med

TC2N inhibits distant metastasis and stemness of breast cancer via blocking fatty acid synthesis

Authors - Xiang-Lin Hao
humanWB

Virol Sin

African Swine Fever Virus MGF360-12L Inhibits Type I Interferon Production by Blocking the Interaction of Importin α and NF-κB Signaling Pathway.

Authors - Yisha Zhuo
humanWB

PLoS Pathog

Phospholipid scramblase 1 interacts with influenza A virus NP, impairing its nuclear import and thereby suppressing virus replication.

Authors - Weiyu Luo
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