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  • Featured Product
  • KD/KO Validated

Importin Beta Polyclonal antibody

Importin Beta Polyclonal Antibody for WB, IHC, IF/ICC, FC (Intra), ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, FC (Intra), ELISA and More (3)

Conjugate

Unconjugated

Cat no : 10077-1-AP

Print datasheet

Synonyms

KPNB1, Importin-90, Importin subunit beta-1, Importin subunit beta 1, Importin 90

验证数据展示

  • WB analysis using 10077-1-AP

    WB analysis using 10077-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 10077-1-AP (Importin Beta antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • IHC staining of human colon cancer using 10077-1-AP

    IHC staining of human colon cancer using 10077-1-AP

    Immunohistochemical analysis of paraffin-embedded human colon cancer using 10077-1-AP (Importin beta antibody) at dilution of 1:50 (under 10x lens).

    Ready-to-use IHC kits available with KPNB1 antibody included
  • IF Staining of HeLa using 10077-1-AP

    IF Staining of HeLa using 10077-1-AP

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using Importin Beta antibody (10077-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red).

  • FC experiment of Jurkat using 10077-1-AP

    FC experiment of Jurkat using 10077-1-AP

    1X10^6 Jurkat cells were intracellularly stained with 0.4 ug Anti-Human Importin Beta (10077-1-AP) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Isotype Control. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

经过测试的应用

Positive WB detected inA2780 cells, mouse brain tissue, rat brain tissue, HeLa cells, NIH/3T3 cells, C6 cells, HT-1080 cells, PC-12 cells
Positive IHC detected inhuman colon cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHeLa cells
Positive FC (Intra) detected inJurkat cells
Planning an IHC experiment? We recommend our IHCeasy KPNB1 Ready-To-Use IHC Kit. KPNB1 primary antibody included.
Planning an IF experiment? We recommend our CoraLite® Plus 488 conjugated versions of this antibody.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:4000
Immunohistochemistry (IHC)IHC : 1:20-1:200
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

10077-1-AP targets Importin Beta in WB, IHC, IF/ICC, FC (Intra), IP, CoIP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications WB, IHC, IF/ICC, FC (Intra), ELISA Application Description
Cited ApplicationsWB, IHC, IF, IP, CoIP
Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse
Immunogen Importin Beta fusion protein Ag0122 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name karyopherin (importin) beta 1
Synonyms KPNB1, Importin-90, Importin subunit beta-1, Importin subunit beta 1, Importin 90
Calculated Molecular Weight 97 kDa
Observed Molecular Weight 90-97 kDa
GenBank Accession NumberBC003572
Gene Symbol KPNB1
Gene ID (NCBI) 3837
RRIDAB_2133977
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ14974
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Importin beta1, also known karyopherin beta or KPNB1, is a nuclear transport receptor that plays a key role in the nuclear import process. Importin beta1 and importin alpha work in concert to transport cargo into the nucleus, functioning as an essential component of the classical nuclear localization signal (NLS) pathway. Elevated expression of importin beta1 has been found in cervical tumors. (22125623)

实验方案

Product Specific Protocols
WB protocol for Importin Beta antibody 10077-1-APDownload protocol
IHC protocol for Importin Beta antibody 10077-1-APDownload protocol
IF protocol for Importin Beta antibody 10077-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB,IP

Cell Res

Neddylation of PTEN regulates its nuclear import and promotes tumor development.

Authors - Ping Xie
View Article
WB

Cell Rep

CGG repeats in the human FMR1 gene regulate mRNA localization and cellular stress in developing neurons

Authors - Carissa L Sirois
View Article
humanWB

Oncogene

KPNB1 inhibition disrupts proteostasis and triggers unfolded protein response-mediated apoptosis in glioblastoma cells.

Authors - Zhi-Chuan Zhu
  • KD Validated
View Article
humanWB,IP

J Transl Med

TC2N inhibits distant metastasis and stemness of breast cancer via blocking fatty acid synthesis

Authors - Xiang-Lin Hao
View Article
humanWB

Virol Sin

African Swine Fever Virus MGF360-12L Inhibits Type I Interferon Production by Blocking the Interaction of Importin α and NF-κB Signaling Pathway.

Authors - Yisha Zhuo
View Article
humanWB

PLoS Pathog

Phospholipid scramblase 1 interacts with influenza A virus NP, impairing its nuclear import and thereby suppressing virus replication.

Authors - Weiyu Luo
View Article