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验证数据展示

  • IF Staining of HeLa using CL488-10077

    IF Staining of HeLa using CL488-10077

    Immunofluorescent analysis of (-20°C Methanol) fixed HeLa cells using CoraLite® Plus 488 Importin Beta antibody (CL488-10077) at dilution of 1:200.

  • FC experiment of Jurkat using CL488-10077

    FC experiment of Jurkat using CL488-10077

    1X10^6 Jurkat cells were intracellularly stained with 0.8 ug CoraLite® Plus 488 Anti-Human Importin Beta (CL488-10077) (red), or 0.8 ug Isotype Control. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

  • Featured Product
  • KD/KO验证

CoraLite® Plus 488-conjugated Importin Beta Polyclonal antibody

Importin Beta Polyclonal Antibody for IF/ICC, FC (Intra)
Cat No. CL488-10077

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

IF/ICC, FC (Intra)

别名

KPNB1, Importin-90, Importin subunit beta-1, Importin subunit beta 1, Importin 90

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  CoraLite® Plus 488
规格: 

-/ -

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经过测试的应用

Positive IF/ICC detected inHeLa cells
Positive FC (Intra) detected inJurkat cells
Planning an IHC experiment? We recommend our IHCeasy KPNB1 Ready-To-Use IHC Kit. KPNB1 primary antibody included.
For other applications, we recommend the unconjugated version of this antibody, 10077-1-AP

推荐稀释比

应用推荐稀释比
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.80 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL488-10077 targets Importin Beta in IF/ICC, FC (Intra) applications and shows reactivity with human, mouse, rat samples.

经测试应用 IF/ICC, FC (Intra) Application Description
经测试反应性 human, mouse, rat
免疫原 Importin Beta fusion protein Ag0122 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 karyopherin (importin) beta 1
别名 KPNB1, Importin-90, Importin subunit beta-1, Importin subunit beta 1, Importin 90
计算分子量 97 kDa
观测分子量 90-97 kDa
GenBank蛋白编号BC003572
基因名称 KPNB1
Gene ID (NCBI) 3837
RRIDAB_3083861
偶联类型 CoraLite® Plus 488 Fluorescent Dye
最大激发/发射波长493 nm / 522 nm
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ14974
储存缓冲液 PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA , pH 7.3
储存条件Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Importin beta1, also known karyopherin beta or KPNB1, is a nuclear transport receptor that plays a key role in the nuclear import process. Importin beta1 and importin alpha work in concert to transport cargo into the nucleus, functioning as an essential component of the classical nuclear localization signal (NLS) pathway. Elevated expression of importin beta1 has been found in cervical tumors. (22125623)

实验方案

Product Specific Protocols
IF protocol for CL Plus 488 Importin Beta antibody CL488-10077Download protocol
Standard Protocols
Click here to view our Standard Protocols