• All
  • Primary Antibodies
  • Conjugated Antibodies for IF
  • Conjugated Antibodies for FC
  • Secondary Antibodies
  • Antibody Labeling KitsNew
  • ELISA Kits
  • IHC Kits
  • Magnetic Cell Separation Kits
  • Cytokines & Growth Factors
  • Neutralizing/activating Antibodies
  • Nanobody-based Reagents
  • Accessory Products and Kits
  • Fusion Proteins
×
×
Host
0
Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
0
Compatible Species
0
Conjugate
0

验证数据展示

  • WB analysis using 67266-1-Ig

    WB analysis using 67266-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 67266-1-Ig (KRAS-2A-specific antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

  • WB analysis using 67266-1-Ig

    WB analysis using 67266-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 67266-1-Ig (KRAS-2A-specific antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated GAPDH Monoclonal antibody (HRP-60004) as loading control.

  • IF Staining of HeLa using 67266-1-Ig

    IF Staining of HeLa using 67266-1-Ig

    Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using 67266-1-Ig (KRAS-2A-specific antibody) at dilution of 1:100 and CoraLite488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

View 3D epitope

KRAS-4A-specific Monoclonal antibody

KRAS-4A-specific Monoclonal Antibody for WB, IF/ICC, ELISA
Cat No. 67266-1-Ig

Print datasheet

CloneNo. 3C8F5

Host / Isotype

Mouse / IgG2a

Reactivity

human, mouse, rat, pig

Applications

WB, IF/ICC, ELISA

Synonyms

KRAS-4A, KRAS, pan RAS, EC:3.6.5.2, c-K-ras

Formulation:  PBS and Azide
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Unconjugated

Size: 
50 μL
100 μL
150 μL

¥2200/ 100 μL

浓度: 1700 ug/ml

点击咨询售前客服

点击咨询售后客服

经过测试的应用

Positive WB detected inHeLa cells, HEK-293 cells, MCF-7 cells, SH-SY5Y cells, PC-12 cells, Neuro-2a cells
Positive IF/ICC detected inHeLa cells

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:5000
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

67266-1-Ig targets KRAS-4A-specific in WB, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat, pig samples.

Tested Applications WB, IF/ICC, ELISA Application Description
Tested Reactivity human, mouse, rat, pig
Immunogen Peptide 种属同源性预测
Host / Isotype Mouse / IgG2a
Class Monoclonal
Type Antibody
Full Name v-Ki-ras2 Kirsten rat sarcoma viral oncogene homolog
Synonyms KRAS-4A, KRAS, pan RAS, EC:3.6.5.2, c-K-ras
Calculated Molecular Weight 21 kDa
Observed Molecular Weight 22 kDa
GenBank Accession NumberNM_033360
Gene Symbol KRAS
Gene ID (NCBI) 3845
RRIDAB_2882537
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDP01116
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

RAS (derived from 'RAt Sarcoma' virus) gene family includes KRAS, HRAS, and NRAS. These genes encode very similar 21 kDa proteins and are homologs of rodent sarcoma virus genes that have transforming abilities. KRAS has two isoforms, 4A and 4B. This antibody is specific for KRAS-4A, which is also named as KRAS-2A.

实验方案

Product Specific Protocols
WB protocol for KRAS-4A-specific antibody 67266-1-IgDownload protocol
IF protocol for KRAS-4A-specific antibody 67266-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols