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验证数据展示

  • WB analysis using 67266-1-Ig

    WB analysis using 67266-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 67266-1-Ig (KRAS-2A-specific antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

  • WB analysis using 67266-1-Ig

    WB analysis using 67266-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 67266-1-Ig (KRAS-2A-specific antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated GAPDH Monoclonal antibody (HRP-60004) as loading control.

  • IF Staining of HeLa using 67266-1-Ig

    IF Staining of HeLa using 67266-1-Ig

    Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using 67266-1-Ig (KRAS-2A-specific antibody) at dilution of 1:100 and CoraLite488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

KRAS-4A-specific Monoclonal antibody

KRAS-4A-specific Monoclonal Antibody for WB, IF/ICC, ELISA
Cat No. 67266-1-Ig

产品说明书

CloneNo. 3C8F5

宿主/亚型

Mouse / IgG2a

种属反应性

human, mouse, rat, pig

应用

WB, IF/ICC, ELISA

别名

KRAS-4A, KRAS, pan RAS, EC:3.6.5.2, c-K-ras

缓冲液配方:  PBS and Azide
PBS and Azide
PBS Only
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inHeLa cells, HEK-293 cells, MCF-7 cells, SH-SY5Y cells, PC-12 cells, Neuro-2a cells
Positive IF/ICC detected inHeLa cells

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:1000-1:5000
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

67266-1-Ig targets KRAS-4A-specific in WB, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat, pig samples.

经测试应用 WB, IF/ICC, ELISA Application Description
经测试反应性 human, mouse, rat, pig
免疫原 Peptide 种属同源性预测
宿主/亚型 Mouse / IgG2a
抗体类别 Monoclonal
产品类型 Antibody
全称 v-Ki-ras2 Kirsten rat sarcoma viral oncogene homolog
别名 KRAS-4A, KRAS, pan RAS, EC:3.6.5.2, c-K-ras
计算分子量 21 kDa
观测分子量 22 kDa
GenBank蛋白编号NM_033360
基因名称 KRAS
Gene ID (NCBI) 3845
RRIDAB_2882537
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purification
UNIPROT IDP01116
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

RAS (derived from 'RAt Sarcoma' virus) gene family includes KRAS, HRAS, and NRAS. These genes encode very similar 21 kDa proteins and are homologs of rodent sarcoma virus genes that have transforming abilities. KRAS has two isoforms, 4A and 4B. This antibody is specific for KRAS-4A, which is also named as KRAS-2A.

实验方案

Product Specific Protocols
WB protocol for KRAS-4A-specific antibody 67266-1-IgDownload protocol
IF protocol for KRAS-4A-specific antibody 67266-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols