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Host
0
Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
0
Compatible Species
0
Conjugate
0

验证数据展示

  • WB analysis using 67266-1-Ig (same clone as 67266-1-PBS)

    WB analysis using 67266-1-Ig (same clone as 67266-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 67266-1-Ig (KRAS-2A-specific antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67266-1-PBS in a different storage buffer formulation.

  • WB analysis using 67266-1-Ig (same clone as 67266-1-PBS)

    WB analysis using 67266-1-Ig (same clone as 67266-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 67266-1-Ig (KRAS-2A-specific antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated GAPDH Monoclonal antibody (HRP-60004) as loading control. This data was developed using the same antibody clone with 67266-1-PBS in a different storage buffer formulation.

  • IF Staining of HeLa using 67266-1-Ig (same clone as 67266-1-PBS)

    IF Staining of HeLa using 67266-1-Ig (same clone as 67266-1-PBS)

    Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using 67266-1-Ig (KRAS-2A-specific antibody) at dilution of 1:100 and CoraLite488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 67266-1-PBS in a different storage buffer formulation.

KRAS-4A-specific Monoclonal antibody, PBS Only

KRAS-4A-specific Monoclonal Antibody for WB, IF/ICC, ELISA
Cat No. 67266-1-PBS
CloneNo. 3C8F5

Host / Isotype

Mouse / IgG2a

Reactivity

human, mouse, rat, pig

Applications

WB, IF/ICC, ELISA

Synonyms

KRAS-4A, KRAS, pan RAS, EC:3.6.5.2, c-K-ras

Formulation:  PBS Only
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Size: 

-/ -

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产品信息

67266-1-PBS targets KRAS-4A-specific in WB, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat, pig samples.

Tested Applications WB, IF/ICC, ELISA Application Description
Tested Reactivity human, mouse, rat, pig
Immunogen Peptide 种属同源性预测
Host / Isotype Mouse / IgG2a
Class Monoclonal
Type Antibody
Full Name v-Ki-ras2 Kirsten rat sarcoma viral oncogene homolog
Synonyms KRAS-4A, KRAS, pan RAS, EC:3.6.5.2, c-K-ras
Calculated Molecular Weight 21 kDa
Observed Molecular Weight 22 kDa
GenBank Accession NumberNM_033360
Gene Symbol KRAS
Gene ID (NCBI) 3845
RRIDAB_2882537
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDP01116
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

RAS (derived from 'RAt Sarcoma' virus) gene family includes KRAS, HRAS, and NRAS. These genes encode very similar 21 kDa proteins and are homologs of rodent sarcoma virus genes that have transforming abilities. KRAS has two isoforms, 4A and 4B. This antibody is specific for KRAS-4A, which is also named as KRAS-2A.