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Cytokeratin 13 Polyclonal antibody

Cytokeratin 13 Polyclonal Antibody for WB, IHC, IF/ICC, FC (Intra), IP, ELISA
Cat No. 10164-2-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse and More (1)

应用

WB, IHC, IF/ICC, FC (Intra), IP, ELISA and More (1)

KRT13, Cytokeratin-13, Cytokeratin,Keratin, ck17, CK-13

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
规格: 

-/ -


经过测试的应用

Positive WB detected inA431 cells, mouse skin tissue
Positive IP detected inA431 cells
Positive IHC detected inhuman cervical cancer tissue, human oesophagus tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHaCaT cells, A431 cells
Positive FC (Intra) detected inA431 cells
Planning an IHC experiment? We recommend our IHCeasy KRT13 Ready-To-Use IHC Kit. KRT13 primary antibody included.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:5000-1:10000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

10164-2-AP targets Cytokeratin 13 in WB, IHC, IF/ICC, FC (Intra), IP, ELISA applications and shows reactivity with human, mouse samples.

经测试应用 WB, IHC, IF/ICC, FC (Intra), IP, ELISA Application Description
文献引用应用WB, IHC, IF
经测试反应性 human, mouse
文献引用反应性human, mouse, monkey
免疫原 Cytokeratin 13 fusion protein Ag0217 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 keratin 13
别名 KRT13, Cytokeratin-13, Cytokeratin,Keratin, ck17, CK-13
计算分子量 50 kDa
观测分子量 50 kDa
GenBank蛋白编号BC002661
基因名称 Cytokeratin 13
Gene ID (NCBI) 3860
RRIDAB_2134679
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDP13646
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Keratin 13 is a member of the keratin family. The keratins are intermediate filament proteins responsible for the structural integrity of epithelial cells and are subdivided into cytokeratins and hair keratins. Most of the type I cytokeratins consist of acidic proteins which are arranged in pairs of heterotypic keratin chains. This type I cytokeratin is paired with keratin 4 and expressed in the suprabasal layers of non-cornified stratified epithelia. Mutations in keratin 13 gene and keratin 4 have been associated with the autosomal dominant disorder White Sponge Nevus. The type I cytokeratins are clustered in a region of chromosome 17q21.2.

实验方案

Product Specific Protocols
WB protocol for Cytokeratin 13 antibody 10164-2-APDownload protocol
IHC protocol for Cytokeratin 13 antibody 10164-2-APDownload protocol
IF protocol for Cytokeratin 13 antibody 10164-2-APDownload protocol
IP protocol for Cytokeratin 13 antibody 10164-2-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
mouseIF

Theranostics

Differential effect of cancer-associated fibroblast-derived extracellular vesicles on cisplatin resistance in oral squamous cell carcinoma via miR-876-3p

Authors - Soo Hyun Kang
humanIF

Elife

Chloride channels regulate differentiation and barrier functions of the mammalian airway.

Authors - Mu He
humanWB

Cell Commun Signal

Liprin-α1 modulates cancer cell signaling by transmembrane protein CD82 in adhesive membrane domains linked to cytoskeleton.

Authors - Henna Pehkonen
humanIF

Am J Respir Cell Mol Biol

Do airway epithelium air-liquid cultures represent the in vivo airway epithelium transcriptome?

Authors - Dvorak Anna A
humanIF

Stem Cells Int

Pyridoxal-5'-Phosphate Promotes Immunomodulatory Function of Adipose-Derived Mesenchymal Stem Cells through Indoleamine 2,3-Dioxygenase-1 and TLR4/NF-κB Pathway.

Authors - Cong Li
humanIHC

Mol Vis

Expression analysis of human pterygium shows a predominance of conjunctival and limbal markers and genes associated with cell migration.

Authors - Jaworski C J CJ
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