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验证数据展示

  • FC experiment of A431 using CL647-10164

    FC experiment of A431 using CL647-10164

    1x10^6 A431 cells were intracellularly stained with 0.8 ug CoraLite® Plus 647-conjugated Cytokeratin 13 Polyclonal antibody (CL647-10164)(red), or 0.8 ug CoraLite® Plus 647-conjugated Rabbit IgG control Rabbit PolyAb (CL647-30000) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

  • Featured Product
  • KD/KO Validated

CoraLite® Plus 647-conjugated Cytokeratin 13 Polyclonal antibody

Cytokeratin 13 Polyclonal Antibody for FC (Intra)
Cat No. CL647-10164

Print datasheet

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

FC (Intra)

Synonyms

KRT13, Cytokeratin-13, Cytokeratin,Keratin, ck17, CK-13

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  CoraLite® Plus 647
Size: 

-/ -

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经过测试的应用

Positive FC (Intra) detected inA431 cells
Planning an IHC experiment? We recommend our IHCeasy KRT13 Ready-To-Use IHC Kit. KRT13 primary antibody included.

推荐稀释比

ApplicationDilution
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.80 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL647-10164 targets Cytokeratin 13 in FC (Intra) applications and shows reactivity with human, mouse samples.

Tested Applications FC (Intra) Application Description
Tested Reactivity human, mouse
Immunogen Cytokeratin 13 fusion protein Ag0217 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name keratin 13
Synonyms KRT13, Cytokeratin-13, Cytokeratin,Keratin, ck17, CK-13
Calculated Molecular Weight 50 kDa
Observed Molecular Weight 50 kDa
GenBank Accession NumberBC002661
Gene Symbol Cytokeratin 13
Gene ID (NCBI) 3860
RRIDAB_2934810
Conjugate CoraLite® Plus 647 Fluorescent Dye
Excitation/Emission Maxima Wavelengths654 nm / 674 nm
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDP13646
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Keratin 13 is a member of the keratin family. The keratins are intermediate filament proteins responsible for the structural integrity of epithelial cells and are subdivided into cytokeratins and hair keratins. Most of the type I cytokeratins consist of acidic proteins which are arranged in pairs of heterotypic keratin chains. This type I cytokeratin is paired with keratin 4 and expressed in the suprabasal layers of non-cornified stratified epithelia. Mutations in keratin 13 gene and keratin 4 have been associated with the autosomal dominant disorder White Sponge Nevus. The type I cytokeratins are clustered in a region of chromosome 17q21.2.

实验方案

Product Specific Protocols
FC protocol for CL Plus 647 Cytokeratin 13 antibody CL647-10164Download protocol
Standard Protocols
Click here to view our Standard Protocols