验证数据展示
经过测试的应用
| Positive WB detected in | HeLa cells, A375 cells, MCF-7 cells |
| Positive IHC detected in | human oesophagus cancer tissue, human bowen disease, human cervical cancer tissue, human skin tissue, mouse skin tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | A431 cells |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:5000-1:50000 |
| Immunohistochemistry (IHC) | IHC : 1:500-1:2000 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
发表文章中的应用
| WB | See 3 publications below |
| IF | See 2 publications below |
产品信息
17265-1-AP targets Cytokeratin 16 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse samples.
| 经测试应用 | WB, IHC, IF/ICC, ELISA Application Description |
| 文献引用应用 | WB, IF |
| 经测试反应性 | human, mouse |
| 文献引用反应性 | human, mouse |
| 免疫原 | Cytokeratin 16 fusion protein Ag10999 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | keratin 16 |
| 别名 | KRT16, Keratin16, keratin 16, Cytokeratin,Keratin, CK-16 |
| 计算分子量 | 473 aa, 51 kDa |
| 观测分子量 | 48 kDa |
| GenBank蛋白编号 | BC039169 |
| 基因名称 | Cytokeratin 16 |
| Gene ID (NCBI) | 3868 |
| RRID | AB_2132894 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | P08779 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
Keratins are a large family of proteins that form the intermediate filament cytoskeleton of epithelial cells, which are classified into two major sequence types. Type I keratins are a group of acidic intermediate filament proteins, including K9-K23, and the hair keratins Ha1-Ha8. Type II keratins are the basic or neutral courterparts to the acidic type I keratins, including K1-K8, and the hair keratins, Hb1-Hb6. Keratin 16 is a type I cytokeratin. It is paired with keratin 6 in a number of epithelial tissues, including nail bed, esophagus, tongue, and hair follicles.
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for Cytokeratin 16 antibody 17265-1-AP | Download protocol |
| IHC protocol for Cytokeratin 16 antibody 17265-1-AP | Download protocol |
| IF protocol for Cytokeratin 16 antibody 17265-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Phytother Res Epigallocatechin-3-gallate promotes wound healing response in diabetic mice by activating keratinocytes and promoting re-epithelialization | ||
Int J Clin Exp Pathol Wnt/β-catenin signaling regulates pathogenesis of human middle ear cholesteatoma | ||
Discov Oncol Identification of KRT16 and ANXA10 as cell cycle regulation genes for lung adenocarcinoma based on self-transcriptome sequencing of surgical samples and TCGA public data mining | ||
Front Pharmacol Protective effect of Saussurea involucrata polysaccharide against skin dryness induced by ultraviolet radiation |
