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验证数据展示

  • IF Staining of A431 using CL488-17265

    IF Staining of A431 using CL488-17265

    Immunofluorescent analysis of (-20°C Methanol) fixed A431 cells using CoraLite® Plus 488 Cytokeratin 16 antibody (CL488-17265) at dilution of 1:200.

CoraLite® Plus 488-conjugated Cytokeratin 16 Polyclonal antibody

Cytokeratin 16 Polyclonal Antibody for IF/ICC
Cat No. CL488-17265

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse

应用

IF/ICC

别名

CK 16, CK16, Cytokeratin 16, K16, K1CP, keratin 16, KRT16, KRT16A, NEPPK

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  CoraLite® Plus 488
规格: 

-/ -

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经过测试的应用

Positive IF/ICC detected inA431 cells
Planning an IHC experiment? We recommend our IHCeasy KRT16 Ready-To-Use IHC Kit. KRT16 primary antibody included.
For other applications, we recommend the unconjugated version of this antibody, 17265-1-AP

推荐稀释比

应用推荐稀释比
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL488-17265 targets Cytokeratin 16 in IF/ICC applications and shows reactivity with human, mouse samples.

经测试应用 IF/ICC Application Description
经测试反应性 human, mouse
免疫原 Cytokeratin 16 fusion protein Ag10999 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 keratin 16
别名 CK 16, CK16, Cytokeratin 16, K16, K1CP, keratin 16, KRT16, KRT16A, NEPPK
计算分子量 473 aa, 51 kDa
观测分子量 48 kDa
GenBank蛋白编号BC039169
基因名称 Cytokeratin 16
Gene ID (NCBI) 3868
RRIDAB_3672668
偶联类型 CoraLite® Plus 488 Fluorescent Dye
最大激发/发射波长493 nm / 522 nm
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDP08779
储存缓冲液 PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA , pH 7.3
储存条件Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Keratins are a large family of proteins that form the intermediate filament cytoskeleton of epithelial cells, which are classified into two major sequence types. Type I keratins are a group of acidic intermediate filament proteins, including K9-K23, and the hair keratins Ha1-Ha8. Type II keratins are the basic or neutral courterparts to the acidic type I keratins, including K1-K8, and the hair keratins, Hb1-Hb6. Keratin 16 is a type I cytokeratin. It is paired with keratin 6 in a number of epithelial tissues, including nail bed, esophagus, tongue, and hair follicles.

实验方案

Product Specific Protocols
IF protocol for CL Plus 488 Cytokeratin 16 antibody CL488-17265Download protocol
Standard Protocols
Click here to view our Standard Protocols