验证数据展示
经过测试的应用
| Positive WB detected in | HeLa cells, A431 cells |
| Positive IP detected in | HeLa cells |
| Positive IHC detected in | human cervical cancer tissue, human lung cancer tissue, human colon cancer tissue, human pancreas cancer tissue, human skin tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | HeLa cells |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:500-1:3000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:20-1:200 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:200-1:800 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
18502-1-AP targets Cytokeratin 17 in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.
| 经测试应用 | WB, IHC, IF/ICC, IP, ELISA Application Description |
| 文献引用应用 | WB, IHC, IF, ELISA |
| 经测试反应性 | human, mouse, rat |
| 文献引用反应性 | human, mouse, goat |
| 免疫原 | Cytokeratin 17 fusion protein Ag13351 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | keratin 17 |
| 别名 | KRT17, Keratin-17, keratin 17, Cytokeratin-17, Cytokeratin,Keratin |
| 计算分子量 | 48 kDa |
| 观测分子量 | 48 kDa |
| GenBank蛋白编号 | BC011901 |
| 基因名称 | Cytokeratin 17 |
| Gene ID (NCBI) | 3872 |
| RRID | AB_10644296 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | Q04695 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
Keratins are a large family of proteins that form the intermediate filaments that make up the cytoskeleton. Type I keratins are a group of acidic intermediate filament proteins and type II keratins are the basic or neutral counterparts. Keratin 17 is encoded by the KRT17 gene and is a type I cytokeratin found in nail beds, hair follicles, sebaceous glands, and epidermal appendages.
What is the molecular weight of cytokeratin 17?
The molecular weight of cytokeratin 17 is approximately 49 kDa.
What is the function of cytokeratin 17?
Cytokeratin 17 plays a role in the formation and maintenance of epidermal appendages, especially in determining the shape and orientation of hair. It is required for the maintenance of the anagen (growth) state of hair follicles by modulating the function of TNF-alpha for hair cycling and is also involved in tissue repair (PMID: 16702408).
What is the cellular localization of cytokeratin 17?
Cytokeratin 17 expression is located in the cytoplasm.
What is the tissue specificity of cytokeratin 17?
Cytokeratin 17 expression is found in the outer root sheath and medulla regions of the hair follicle, particularly in the eyebrow, beard, nail bed epithelium, basal cells of the oral epithelium, and sweat and mammary glands (PMID: 2451629). Expression can also be found in epithelial layers of the prostate and urinary bladder and cambial cells of the sebaceous gland at the protein level.
What is the role of cytokeratin 17 in disease?
Mutations in the KRT17 gene are a cause of Jackson-Lawler type pachyonychia congenita type 2 (PC2); an autosomal dominant ectodermal dysplasia characterized by hypertrophic nail dystrophy (PMID: 7539673). Defects in KRT17 also lead to steatocystoma multiplex (SM) (PMID: 9767294). Cytokeratin 17 acts as an autoantigen in the immunopathogenesis of psoriasis (PMID: 23722817).
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for Cytokeratin 17 antibody 18502-1-AP | Download protocol |
| IHC protocol for Cytokeratin 17 antibody 18502-1-AP | Download protocol |
| IF protocol for Cytokeratin 17 antibody 18502-1-AP | Download protocol |
| IP protocol for Cytokeratin 17 antibody 18502-1-AP | Download protocol |
| FC protocol for Cytokeratin 17 antibody 18502-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
J Anim Sci Biotechnol Establishment of goat mammary organoid cultures modeling the mammary gland development and lactation | ||
J Endocr Soc KRT17 From Keratinocytes With High Glucose Stimulation Inhibit Dermal Fibroblasts Migration Through Integrin α11 | ||
Research (Wash D C) Aggregation-Induced Emission-Active Photosensitizer-Mediated Photodynamic Therapy for Anti-Psoriasis | ||
J Inflamm Res CORM-3 Inhibits the Inflammatory Response of Human Periodontal Ligament Fibroblasts Stimulated by LPS and High Glucose |
