CoraLite® Plus 488-conjugated Cytokeratin 17 Polyclonal antibody

Cytokeratin 17 Polyclonal Antibody for IF/ICC

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

IF/ICC

Conjugate

CoraLite® Plus 488 Fluorescent Dye

Cat no : CL488-18502

Print datasheet

Synonyms

39.1, Cell and organelle markers, CK 17, Cytokeratin 17, Cytoskeleton Marker, K17, keratin 17, KRT17, PC, PC2, PCHC1



经过测试的应用

Positive IF/ICC detected inHeLa cells
Planning an IHC experiment? We recommend our IHCeasy KRT17 Ready-To-Use IHC Kit. KRT17 primary antibody included.
For other applications, we recommend the unconjugated version of this antibody, 18502-1-AP

推荐稀释比

ApplicationDilution
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL488-18502 targets Cytokeratin 17 in IF/ICC applications and shows reactivity with human, mouse, rat samples.

Tested Applications IF/ICC Application Description
Tested Reactivity human, mouse, rat
Immunogen Cytokeratin 17 fusion protein Ag13351 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name keratin 17
Synonyms 39.1, Cell and organelle markers, CK 17, Cytokeratin 17, Cytoskeleton Marker, K17, keratin 17, KRT17, PC, PC2, PCHC1
Calculated Molecular Weight 48 kDa
Observed Molecular Weight 48 kDa
GenBank Accession NumberBC011901
Gene Symbol Cytokeratin 17
Gene ID (NCBI) 3872
Conjugate CoraLite® Plus 488 Fluorescent Dye
Excitation/Emission Maxima Wavelengths493 nm / 522 nm
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ04695
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Keratins are a large family of proteins that form the intermediate filaments that make up the cytoskeleton. Type I keratins are a group of acidic intermediate filament proteins and type II keratins are the basic or neutral counterparts. Keratin 17 is encoded by the KRT17 gene and is a type I cytokeratin found in nail beds, hair follicles, sebaceous glands, and epidermal appendages.

 What is the molecular weight of cytokeratin 17?

The molecular weight of cytokeratin 17 is approximately 49 kDa.

 What is the function of cytokeratin 17?

Cytokeratin 17 plays a role in the formation and maintenance of epidermal appendages, especially in determining the shape and orientation of hair. It is required for the maintenance of the anagen (growth) state of hair follicles by modulating the function of TNF-alpha for hair cycling and is also involved in tissue repair (PMID: 16702408).

 What is the cellular localization of cytokeratin 17?

Cytokeratin 17 expression is located in the cytoplasm.

 What is the tissue specificity of cytokeratin 17?

Cytokeratin 17 expression is found in the outer root sheath and medulla regions of the hair follicle, particularly in the eyebrow, beard, nail bed epithelium, basal cells of the oral epithelium, and sweat and mammary glands (PMID: 2451629). Expression can also be found in epithelial layers of the prostate and urinary bladder and cambial cells of the sebaceous gland at the protein level.

 What is the role of cytokeratin 17 in disease?

Mutations in the KRT17 gene are a cause of Jackson-Lawler type pachyonychia congenita type 2 (PC2); an autosomal dominant ectodermal dysplasia characterized by hypertrophic nail dystrophy (PMID: 7539673). Defects in KRT17 also lead to steatocystoma multiplex (SM) (PMID: 9767294). Cytokeratin 17 acts as an autoantigen in the immunopathogenesis of psoriasis (PMID: 23722817).


实验方案

Product Specific Protocols
IF protocol for CL Plus 488 Cytokeratin 17 antibody CL488-18502Download protocol
Standard Protocols
Click here to view our Standard Protocols