Cytokeratin 20 Recombinant antibody

Cytokeratin 20 Uni-rAbTM Recombinant Antibody for WB, IHC, IF/ICC, FC (Intra), ELISA
Cat No. 82428-1-RR

产品说明书

CloneNo. 2H10

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat, pig

应用

WB, IHC, IF/ICC, FC (Intra), ELISA

KRT20, keratin 20, K20, Cytokeratin-20, CK-20

缓冲液配方:  PBS and Azide
PBS and Azide
PBS Only
偶联物:  Unconjugated
Unconjugated
CoraLite® Plus 488
规格: 

-/ -


经过测试的应用

Positive WB detected inHT-29 cells, COLO 320 cells, pig colon tissue, mouse colon tissue, rat colon tissue
Positive IHC detected inhuman colon cancer tissue, human appendicitis tissue, human colon tissue, human urothelial carcinoma tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHT-29 cells
Positive FC (Intra) detected inHT-29 cells
Planning an IHC experiment? We recommend our IHCeasy KRT20 Ready-To-Use IHC Kit. KRT20 primary antibody included.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:5000-1:50000
Immunohistochemistry (IHC)IHC : 1:3000-1:12000
Immunofluorescence (IF)/ICCIF/ICC : 1:500-1:2000
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

82428-1-RR targets Cytokeratin 20 in WB, IHC, IF/ICC, FC (Intra), ELISA applications and shows reactivity with human, mouse, rat, pig samples.

经测试应用 WB, IHC, IF/ICC, FC (Intra), ELISA Application Description
经测试反应性 human, mouse, rat, pig
免疫原 Cytokeratin 20 fusion protein Ag11308 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 keratin 20
别名 KRT20, keratin 20, K20, Cytokeratin-20, CK-20
计算分子量 424 aa, 48 kDa
观测分子量46 kDa
GenBank蛋白编号BC031559
基因名称 Cytokeratin 20
Gene ID (NCBI) 54474
RRIDAB_3086478
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purification
UNIPROT IDP35900
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Keratins are a large family of proteins that form the intermediate filament cytoskeleton of epithelial cells, which are classified into two major sequence types. Type I keratins are a group of acidic intermediate filament proteins, including K9-K23, and the hair keratins Ha1-Ha8. Type II keratins are the basic or neutral courterparts to the acidic type I keratins, including K1-K8, and the hair keratins, Hb1-Hb6. Keratin 20 is a type I cytokeratin. It is a major cellular protein of mature enterocytes and goblet cells and is specifically found in the gastric and intestinal mucosa.

实验方案

Product Specific Protocols
WB protocol for Cytokeratin 20 antibody 82428-1-RRDownload protocol
IHC protocol for Cytokeratin 20 antibody 82428-1-RRDownload protocol
IF protocol for Cytokeratin 20 antibody 82428-1-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols
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