验证数据展示
经过测试的应用
Positive IF/ICC detected in | HT-29 cells |
Positive FC (Intra) detected in | HT-29 cells |
Positive FC detected in | HT-29 cells |
推荐稀释比
应用 | 推荐稀释比 |
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Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
Flow Cytometry (FC) (INTRA) | FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension |
Flow Cytometry (FC) | FC : 0.40 ug per 10^6 cells in a 100 µl suspension |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
产品信息
CL488-82428 targets Cytokeratin 20 in IF/ICC, FC (Intra) applications and shows reactivity with Human, mouse, rat, pig samples.
经测试应用 | IF/ICC, FC (Intra) Application Description |
经测试反应性 | Human, mouse, rat, pig |
免疫原 | Cytokeratin 20 fusion protein Ag11308 种属同源性预测 |
宿主/亚型 | Rabbit / IgG |
抗体类别 | Recombinant |
产品类型 | Antibody |
全称 | keratin 20 |
别名 | CK 20, CK20, Cytokeratin 20, K20, keratin 20, KRT20, KRT21, Protein IT |
计算分子量 | 424 aa, 48 kDa |
观测分子量 | 46 kDa |
GenBank蛋白编号 | BC031559 |
基因名称 | Cytokeratin 20 |
Gene ID (NCBI) | 54474 |
RRID | AB_3084514 |
偶联类型 | CoraLite® Plus 488 Fluorescent Dye |
最大激发/发射波长 | 493 nm / 522 nm |
形式 | Liquid |
纯化方式 | Protein A purification |
UNIPROT ID | P35900 |
储存缓冲液 | PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA , pH 7.3 |
储存条件 | Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
Keratins are a large family of proteins that form the intermediate filament cytoskeleton of epithelial cells, which are classified into two major sequence types. Type I keratins are a group of acidic intermediate filament proteins, including K9-K23, and the hair keratins Ha1-Ha8. Type II keratins are the basic or neutral courterparts to the acidic type I keratins, including K1-K8, and the hair keratins, Hb1-Hb6. Keratin 20 is a type I cytokeratin. It is a major cellular protein of mature enterocytes and goblet cells and is specifically found in the gastric and intestinal mucosa.
实验方案
Product Specific Protocols | |
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IF protocol for CL Plus 488 Cytokeratin 20 antibody CL488-82428 | Download protocol |
FC protocol for CL Plus 488 Cytokeratin 20 antibody CL488-82428 | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |