CoraLite® Plus 488-conjugated Cytokeratin 20 Recombinant antibody
Cytokeratin 20 Uni-rAbTM Recombinant Antibody for IF/ICC, FC (Intra)
Host / Isotype
Rabbit / IgG
Reactivity
Human, mouse, rat, pig
Applications
IF/ICC, FC (Intra)
Conjugate
CoraLite® Plus 488 Fluorescent Dye
CloneNo.
2H10
验证数据展示
经过测试的应用
Positive IF/ICC detected in | HT-29 cells |
Positive FC (Intra) detected in | HT-29 cells |
Positive FC detected in | HT-29 cells |
推荐稀释比
Application | Dilution |
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Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
Flow Cytometry (FC) (INTRA) | FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension |
Flow Cytometry (FC) | FC : 0.40 ug per 10^6 cells in a 100 µl suspension |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
产品信息
CL488-82428 targets Cytokeratin 20 in IF/ICC, FC (Intra) applications and shows reactivity with Human, mouse, rat, pig samples.
Tested Applications | IF/ICC, FC (Intra) Application Description |
Tested Reactivity | Human, mouse, rat, pig |
Immunogen | Cytokeratin 20 fusion protein Ag11308 种属同源性预测 |
Host / Isotype | Rabbit / IgG |
Class | Recombinant |
Type | Antibody |
Full Name | keratin 20 |
Synonyms | CK 20, CK20, Cytokeratin 20, K20, keratin 20, KRT20, KRT21, Protein IT |
Calculated Molecular Weight | 424 aa, 48 kDa |
Observed Molecular Weight | 46 kDa |
GenBank Accession Number | BC031559 |
Gene Symbol | Cytokeratin 20 |
Gene ID (NCBI) | 54474 |
Conjugate | CoraLite® Plus 488 Fluorescent Dye |
Excitation/Emission Maxima Wavelengths | 493 nm / 522 nm |
Form | Liquid |
Purification Method | Protein A purification |
UNIPROT ID | P35900 |
Storage Buffer | PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3. |
Storage Conditions | Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
实验方案
Product Specific Protocols | |
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IF protocol for CL Plus 488 Cytokeratin 20 antibody CL488-82428 | Download protocol |
FC protocol for CL Plus 488 Cytokeratin 20 antibody CL488-82428 | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |