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验证数据展示

  • IHC staining of human oesophagus cancer using Biotin-16835

    IHC staining of human oesophagus cancer using Biotin-16835

    Immunohistochemical analysis of paraffin-embedded human oesophagus cancer tissue slide using Biotin-16835 (KRT80 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of human oesophagus cancer using Biotin-16835

    IHC staining of human oesophagus cancer using Biotin-16835

    Immunohistochemical analysis of paraffin-embedded human oesophagus cancer tissue slide using Biotin-16835 (KRT80 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Featured Product
  • KD/KO验证

Biotin-conjugated Cytokeratin 80 Polyclonal antibody

Cytokeratin 80 Polyclonal Antibody for IHC
Cat No. Biotin-16835

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

IHC

别名

KRT80, CK 80, CK-80, Cytokeratin-80, K80

缓冲液配方:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
偶联物:  Biotin
规格价格库存

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经过测试的应用

Positive IHC detected inhuman oesophagus cancer tissue, human colon cancer, human colon cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

应用推荐稀释比
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

Biotin-16835 targets Cytokeratin 80 in IHC applications and shows reactivity with human, mouse, rat samples.

经测试应用 IHC Application Description
经测试反应性 human, mouse, rat
免疫原

CatNo: Ag10498

Product name: Recombinant human KRT80 protein

Source: e coli.-derived, PET28a

Tag: 6*His

Domain: 1-422 aa of BC065180

Sequence: MACRSCVVGFSSLSSCEVTPVGSPRPGTSGWDSCRAPGPGFSSRSLTGCWSAGTISKVTVNPGLLVPLDVKLDPAVQQLKNQEKEEMKALNDKFASLIGKVQALEQRNQLLETRWSFLQGQDSAIFDLGHLYEEYQGRLQEELRKVSQERGQLEANLLQVLEKVEEFRIRYEDEISKRTDMEFTFVQLKKDLDAECLHRTELETKLKSLESFVELMKTIYEQELKDLAAQVKDVSVTVGMDSRCHIDLSGIVEEVKAQYDAVAARSLEEAEAYSRSQLEEQAARSAEYGSSLQSSRSEIADLNVRIQKLRSQILSVKSHCLKLEENIKTAEEQGELAFQDAKTKLAQLEAALQQAKQDMARQLRKYQELMNVKLALDIEIATYRKLVEGEEGRMDSPSATVVSAVQSRCKTAPSLPYPLCSL

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 keratin 80
别名 KRT80, CK 80, CK-80, Cytokeratin-80, K80
计算分子量 422 aa, 47 kDa
观测分子量 47 kDa, 50 kDa
GenBank蛋白编号BC065180
基因名称 KRT80
Gene ID (NCBI) 144501
RRIDAB_2934330
偶联类型 Biotin
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ6KB66
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
IHC protocol for Biotin Cytokeratin 80 antibody Biotin-16835Download protocol
Standard Protocols
Click here to view our Standard Protocols
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