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  • Featured Product
  • KD/KO Validated

Biotin-conjugated KRT80 Polyclonal antibody

KRT80 Polyclonal Antibody for IHC

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

IHC

Conjugate

Biotin

Cat no : Biotin-16835

Print datasheet

Synonyms

KB20, K80, Cytokeratin-80, Cytokeratin 80, CK-80

验证数据展示

  • IHC staining of human oesophagus cancer using Biotin-16835

    IHC staining of human oesophagus cancer using Biotin-16835

    Immunohistochemical analysis of paraffin-embedded human oesophagus cancer tissue slide using Biotin-16835 (KRT80 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of human oesophagus cancer using Biotin-16835

    IHC staining of human oesophagus cancer using Biotin-16835

    Immunohistochemical analysis of paraffin-embedded human oesophagus cancer tissue slide using Biotin-16835 (KRT80 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

经过测试的应用

Positive IHC detected inhuman oesophagus cancer tissue, human colon cancer, human colon cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

ApplicationDilution
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

Biotin-16835 targets KRT80 in IHC applications and shows reactivity with human, mouse, rat samples.

Tested Applications IHC Application Description
Tested Reactivity human, mouse, rat
Immunogen KRT80 fusion protein Ag10498 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name keratin 80
Synonyms KB20, K80, Cytokeratin-80, Cytokeratin 80, CK-80
Calculated Molecular Weight 422 aa, 47 kDa
Observed Molecular Weight 47 kDa, 50 kDa
GenBank Accession NumberBC065180
Gene Symbol KRT80
Gene ID (NCBI) 144501
Conjugate Biotin
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ6KB66
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
IHC protocol for Biotin KRT80 antibody Biotin-16835Download protocol
Standard Protocols
Click here to view our Standard Protocols