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验证数据展示

  • IF Staining of HeLa using CL488-11342

    IF Staining of HeLa using CL488-11342

    Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using CoraLite® Plus 488 KRT81 antibody (CL488-11342) at dilution of 1:200, CL594-Phalloidin (red).

  • Featured Product
  • KD/KO验证

CoraLite® Plus 488-conjugated Cytokeratin 81 Polyclonal antibody

Cytokeratin 81 Polyclonal Antibody for IF/ICC
Cat No. CL488-11342

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse

应用

IF/ICC

别名

KRT81, Cytokeratin,Keratin, ghHb1, keratin 81, Keratin-81

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  CoraLite® Plus 488
规格: 

-/ -

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经过测试的应用

Positive IF/ICC detected inHeLa cells
Planning an IHC experiment? We recommend our IHCeasy KRT81 Ready-To-Use IHC Kit. KRT81 primary antibody included.
For other applications, we recommend the unconjugated version of this antibody, 11342-1-AP

推荐稀释比

应用推荐稀释比
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL488-11342 targets Cytokeratin 81 in IF/ICC applications and shows reactivity with human, mouse samples.

经测试应用 IF/ICC Application Description
经测试反应性 human, mouse
免疫原 Cytokeratin 81 fusion protein Ag1890 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 keratin 81
别名 KRT81, Cytokeratin,Keratin, ghHb1, keratin 81, Keratin-81
计算分子量 55 kDa
观测分子量 54 kDa
GenBank蛋白编号BC021241
基因名称 KRT81
Gene ID (NCBI) 3887
RRIDAB_3672491
偶联类型 CoraLite® Plus 488 Fluorescent Dye
最大激发/发射波长493 nm / 522 nm
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ14533
储存缓冲液 PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA , pH 7.3
储存条件Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Keratins are a large family of proteins that form the intermediate filament cytoskeleton of epithelial cells, which are classified into two major sequence types. Type I keratins are a group of acidic intermediate filament proteins, including K9-K23, and the hair keratins Ha1-Ha8. Type II keratins are the basic or neutral courterparts to the acidic type I keratins, including K1-K8, and the hair keratins, Hb1-Hb6. KRT81 is a type II keratin.

实验方案

Product Specific Protocols
IF protocol for CL Plus 488 Cytokeratin 81 antibody CL488-11342Download protocol
Standard Protocols
Click here to view our Standard Protocols