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  • Featured Product
  • KD/KO Validated

CoraLite® Plus 488-conjugated Keratin 81 Polyclonal antibody

Keratin 81 Polyclonal Antibody for IF/ICC

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

IF/ICC

Conjugate

CoraLite® Plus 488 Fluorescent Dye

Cat no : CL488-11342

Print datasheet

Synonyms

ghHb1, ghHkb1, Hair keratin K2.9, Hb 1, HB1, hHAKB2 1, K81, keratin 81, Keratin, hair, basic, 1, Keratin, type II cuticular Hb1, KRT81, KRTHB1, MLN 137, MLN137, Type II hair keratin Hb1, Type II keratin Kb21

验证数据展示

  • IF Staining of HeLa using CL488-11342

    IF Staining of HeLa using CL488-11342

    Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using CoraLite® Plus 488 KRT81 antibody (CL488-11342) at dilution of 1:200, CL594-Phalloidin (red).

经过测试的应用

Positive IF/ICC detected inHeLa cells
Planning an IHC experiment? We recommend our IHCeasy KRT81 Ready-To-Use IHC Kit. KRT81 primary antibody included.
For other applications, we recommend the unconjugated version of this antibody, 11342-1-AP

推荐稀释比

ApplicationDilution
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL488-11342 targets Keratin 81 in IF/ICC applications and shows reactivity with human, mouse samples.

Tested Applications IF/ICC Application Description
Tested Reactivity human, mouse
Immunogen Keratin 81 fusion protein Ag1890 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name keratin 81
Synonyms ghHb1, ghHkb1, Hair keratin K2.9, Hb 1, HB1, hHAKB2 1, K81, keratin 81, Keratin, hair, basic, 1, Keratin, type II cuticular Hb1, KRT81, KRTHB1, MLN 137, MLN137, Type II hair keratin Hb1, Type II keratin Kb21
Calculated Molecular Weight 55 kDa
Observed Molecular Weight 54 kDa
GenBank Accession NumberBC021241
Gene Symbol KRT81
Gene ID (NCBI) 3887
Conjugate CoraLite® Plus 488 Fluorescent Dye
Excitation/Emission Maxima Wavelengths493 nm / 522 nm
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ14533
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Keratins are a large family of proteins that form the intermediate filament cytoskeleton of epithelial cells, which are classified into two major sequence types. Type I keratins are a group of acidic intermediate filament proteins, including K9-K23, and the hair keratins Ha1-Ha8. Type II keratins are the basic or neutral courterparts to the acidic type I keratins, including K1-K8, and the hair keratins, Hb1-Hb6. KRT81 is a type II keratin.

实验方案

Product Specific Protocols
IF protocol for CL Plus 488 Keratin 81 antibody CL488-11342Download protocol
Standard Protocols
Click here to view our Standard Protocols