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Host
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Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
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Compatible Species
0
Conjugate
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验证数据展示

  • FC experiment of C57BL/6 mouse splenocytes using 98224-1-RR (same clone as 98224-1-PBS)

    FC experiment of C57BL/6 mouse splenocytes using 98224-1-RR (same clone as 98224-1-PBS)

    1x10^6 untreated or Con A treated C57BL/6 mouse splenocytes were intracellularly stained with 0.25 ug Anti-Mouse Ki-67 Rabbit Recombinant Antibody (98224-1-RR, Clone: 241768E2) and PE-Conjugated Goat Anti-Rabbit IgG(H+L). Cells were co-stained with CoraLite® Plus 647 Anti-Mouse CD3. Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011). This data was developed using the same antibody clone with 98224-1-PBS in a different storage buffer formulation.

  • FC experiment of C57BL/6 mouse splenocytes using 98224-1-RR (same clone as 98224-1-PBS)

    FC experiment of C57BL/6 mouse splenocytes using 98224-1-RR (same clone as 98224-1-PBS)

    1x10^6 Con A treated C57BL/6 mouse splenocytes were intracellularly stained with 0.25 ug Anti-Mouse Ki-67 Rabbit Recombinant Antibody (98224-1-RR, Clone: 241768E2) (red) or 0.25 ug Rabbit IgG Isotype Control Recombinant Antibody (98136-1-RR, Clone: 240953C9) (blue) and PE-Conjugated Goat Anti-Rabbit IgG(H+L). 1x10^6 untreated C57BL/6 mouse splenocytes were intracellularly stained with 0.25 ug Anti-Mouse Ki-67 Rabbit Recombinant Antibody (98224-1-RR, Clone: 241768E2) (black, dashed) and PE-Conjugated Goat Anti-Rabbit IgG(H+L). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011). This data was developed using the same antibody clone with 98224-1-PBS in a different storage buffer formulation.

Anti-Mouse Ki-67 Rabbit Recombinant Antibody, PBS Only

Ki-67 Uni-rAbTM Recombinant Antibody for FC (Intra)
Cat No. 98224-1-PBS
CloneNo. 241768E2

Host / Isotype

Rabbit / IgG

Reactivity

mouse

Applications

FC (Intra)

Synonyms

Mki67, Ki67, Ki 67, Proliferation related Ki 67 antigen, 241768E2

Formulation:  PBS Only
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Size: 

-/ -

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产品信息

98224-1-PBS targets Ki-67 in FC (Intra) applications and shows reactivity with mouse samples.

Tested Applications FC (Intra) Application Description
Tested Reactivity mouse
Immunogen Recombinant protein 种属同源性预测
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Full Name antigen identified by monoclonal antibody Ki 67
Synonyms Mki67, Ki67, Ki 67, Proliferation related Ki 67 antigen, 241768E2
Calculated Molecular Weight351kDa
GenBank Accession NumberNM_001081117.2
Gene Symbol ki67
Gene ID (NCBI) 17345
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purfication
UNIPROT IDE9PVX6-1
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

The Ki-67 protein (also known as MKI67) is a cellular marker for proliferation. Ki67 is present during all active phases of the cell cycle (G1, S, G2 and M), but is absent in resting cells (G0). Cellular content of Ki-67 protein markedly increases during cell progression through S phase of the cell cycle. Therefore, the nuclear expression of Ki-67 can be evaluated to assess tumor proliferation by immunohistochemistry. It has been demonstrated to be of prognostic value in breast cancer. In head and neck cancer, several studies have reported an association between high proliferative activity and poorer prognosis.