验证数据展示
经过测试的应用
| Positive IF-P detected in | human breast cancer tissue |
For other applications, we recommend the unconjugated version of this antibody, 66269-1-Ig
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Immunofluorescence (IF)-P | IF-P : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
CL488-66269 targets L2HGDH in IF-P applications and shows reactivity with human, pig samples.
| 经测试应用 | IF-P Application Description |
| 经测试反应性 | human, pig |
| 免疫原 | L2HGDH fusion protein Ag8382 种属同源性预测 |
| 宿主/亚型 | Mouse / IgG2a |
| 抗体类别 | Monoclonal |
| 产品类型 | Antibody |
| 全称 | L-2-hydroxyglutarate dehydrogenase |
| 别名 | C14orf160, DURANIN, FLJ12618, L2HGDH |
| 计算分子量 | 463aa,50 kDa; 441aa,49 kDa |
| 观测分子量 | 45 kDa |
| GenBank蛋白编号 | BC006117 |
| 基因名称 | L2HGDH |
| Gene ID (NCBI) | 79944 |
| RRID | AB_2919308 |
| 偶联类型 | CoraLite® Plus 488 Fluorescent Dye |
| 最大激发/发射波长 | 493 nm / 522 nm |
| 形式 | Liquid |
| 纯化方式 | Protein A purification |
| UNIPROT ID | Q9H9P8 |
| 储存缓冲液 | PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3. |
| 储存条件 | Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
L2HGDH(L-2-hydroxyglutarate dehydrogenase, mitochondrial) is also named as duranin, C14orf160 and belongs to the L2HGDH family. The putative L2HGDH is predicted to be targeted to the mitochondria where its mitochondrial targeting sequence is presumably removed(PMID:16005139). Defects in L2HGDH are the cause of L-2-hydroxyglutaric aciduria (L2HGA). It has 2 isoforms produced by alternative splicing with the molecular weight of 50 kDa and 48 kDa. L2HGDH also can be detected as ~45kD due to the 51aa transit peptide cleaved.
实验方案
| Product Specific Protocols | |
|---|---|
| IF protocol for CL Plus 488 L2HGDH antibody CL488-66269 | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |