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Host
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Species Reactivity
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0
Conjugate
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验证数据展示

  • WB analysis using 83495-7-RR (same clone as 83495-7-PBS)

    WB analysis using 83495-7-RR (same clone as 83495-7-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 83495-7-RR (LAMR1/RPSA antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 83495-7-PBS in a different storage buffer formulation.

  • IF Staining of HepG2 using 83495-7-RR (same clone as 83495-7-PBS)

    IF Staining of HepG2 using 83495-7-RR (same clone as 83495-7-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using LAMR1/RPSA antibody (83495-7-RR, Clone: 240461G11 ) at dilution of 1:250 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002). This data was developed using the same antibody clone with 83495-7-PBS in a different storage buffer formulation.

  • FC experiment of MCF-7 using 83495-7-RR (same clone as 83495-7-PBS)

    FC experiment of MCF-7 using 83495-7-RR (same clone as 83495-7-PBS)

    1x10^6 MCF-7 cells were intracellularly stained with 0.25 ug LAMR1/RPSA Recombinant antibody (83495-7-RR, Clone:240461G11) and APC-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 83495-7-PBS in a different storage buffer formulation.

  • Affinity and Kinetic Characterization of 83495-7-RR

    Affinity and Kinetic Characterization of 83495-7-RR

    Biolayer interferometry (BLl) kinetic assays of 83495-7-RR against Human LAMR1,RPSA were performed. The affinity constant is 1.14 nM.

LAMR1/RPSA Recombinant antibody, PBS Only

LAMR1/RPSA Uni-rAbTM Recombinant Antibody for WB, IF/ICC, FC (Intra), ELISA
Cat No. 83495-7-PBS

产品说明书

CloneNo. 240461G11

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse

应用

WB, IF/ICC, FC (Intra), ELISA

别名

LAMR1,RPSA, RPSA, 67LR, 67 kDa laminin receptor, 40S ribosomal protein SA

缓冲液配方:  PBS Only
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格: 

-/ -

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产品信息

83495-7-PBS targets LAMR1/RPSA in WB, IF/ICC, FC (Intra), ELISA applications and shows reactivity with human, mouse samples.

经测试应用 WB, IF/ICC, FC (Intra), ELISA Application Description
经测试反应性 human, mouse
免疫原 LAMR1/RPSA fusion protein Ag6033 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 ribosomal protein SA
别名 LAMR1,RPSA, RPSA, 67LR, 67 kDa laminin receptor, 40S ribosomal protein SA
计算分子量 33 kDa
观测分子量 40 kDa
GenBank蛋白编号BC050688
基因名称 RPSA
Gene ID (NCBI) 3921
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purfication
UNIPROT IDP08865
储存缓冲液 PBS only , pH 7.3
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

The ribosomal protein SA (RPSA), previously named 67 kD laminin receptor(67LR), 37 kD laminin receptor precursor (37LRP) and p40 ribosome-associated protein, is a multifunctional protein, that plays a role in a number of pathological processes, such as cancer and prion diseases. It is overexpressed in various cancer cell lines, and the the level of the laminin receptor transcript is higher in colon carcinoma tissue and lung cancer cell line than their normal counterparts. This antibody is a rabbit polyclonal antibody raised against full length RPSA of human origin. This antibody is specific to RPSA (LAMR1).67LR derives from homo- or hetero- dimerization of a 37 kDa cytosolic precursor (37LRP)], most probably by fatty acid acylation. 37LRP is mostly found in the cytosol and nucleus [10] where it is involved in translational processes and maintenance of nuclear structures, respectively. 67LR is localized at the cell surface and it not only serves as a receptor for LM but also acts as a receptor for elastin, carbohydrates and the cellular prion protein.