LAMR1/RPSA Recombinant antibody

LAMR1/RPSA Uni-rAbTM Recombinant Antibody for WB, IF/ICC, FC (Intra), ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IF/ICC, FC (Intra), ELISA

Conjugate

Unconjugated

CloneNo.

240461G11

Cat no : 83495-7-RR

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Synonyms

LAMR1,RPSA, RPSA, 67LR, 67 kDa laminin receptor, 40S ribosomal protein SA



经过测试的应用

Positive WB detected inHeLa cells, HepG2 cells, NIH/3T3 cells, A549 cells, COLO 320 cells, HEK-293 cells, Jurkat cells
Positive IF/ICC detected inHepG2 cells
Positive FC (Intra) detected inMCF-7 cells

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunofluorescence (IF)/ICCIF/ICC : 1:125-1:500
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

83495-7-RR targets LAMR1/RPSA in WB, IF/ICC, FC (Intra), ELISA applications and shows reactivity with human, mouse samples.

Tested Applications WB, IF/ICC, FC (Intra), ELISA Application Description
Tested Reactivity human, mouse
Immunogen LAMR1/RPSA fusion protein Ag6033 种属同源性预测
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Full Name ribosomal protein SA
Synonyms LAMR1,RPSA, RPSA, 67LR, 67 kDa laminin receptor, 40S ribosomal protein SA
Calculated Molecular Weight 33 kDa
Observed Molecular Weight 40 kDa
GenBank Accession NumberBC050688
Gene Symbol RPSA
Gene ID (NCBI) 3921
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purfication
UNIPROT IDP08865
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

The ribosomal protein SA (RPSA), previously named 67 kD laminin receptor(67LR), 37 kD laminin receptor precursor (37LRP) and p40 ribosome-associated protein, is a multifunctional protein, that plays a role in a number of pathological processes, such as cancer and prion diseases. It is overexpressed in various cancer cell lines, and the the level of the laminin receptor transcript is higher in colon carcinoma tissue and lung cancer cell line than their normal counterparts. This antibody is a rabbit polyclonal antibody raised against full length RPSA of human origin. This antibody is specific to RPSA (LAMR1).67LR derives from homo- or hetero- dimerization of a 37 kDa cytosolic precursor (37LRP)], most probably by fatty acid acylation. 37LRP is mostly found in the cytosol and nucleus [10] where it is involved in translational processes and maintenance of nuclear structures, respectively. 67LR is localized at the cell surface and it not only serves as a receptor for LM but also acts as a receptor for elastin, carbohydrates and the cellular prion protein.

实验方案

Product Specific Protocols
WB protocol for LAMR1/RPSA antibody 83495-7-RRDownload protocol
IF protocol for LAMR1/RPSA antibody 83495-7-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols