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CoraLite® Plus 488-conjugated LAMR1/RPSA Monoclonal antibody

LAMR1/RPSA Monoclonal Antibody for IF/ICC

Host / Isotype

Mouse / IgM

Reactivity

human, mouse, rat

Applications

IF/ICC

Conjugate

CoraLite® Plus 488 Fluorescent Dye

CloneNo.

2A9A1

Cat no : CL488-67324

Print datasheet

Synonyms

LAMR1,RPSA, RPSA, 67LR, 67 kDa laminin receptor, 40S ribosomal protein SA

验证数据展示

  • IF Staining of A549 using CL488-67324

    IF Staining of A549 using CL488-67324

    Immunofluorescent analysis of (-20°C Methanol) fixed A549 cells using CoraLite® Plus 488 LAMR1/RPSA antibody (CL488-67324, Clone: 2A9A1 ) at dilution of 1:200.

  • FC experiment of HepG2 using CL488-67324

    FC experiment of HepG2 using CL488-67324

    1X10^6 HepG2 cells were intracellularly stained with 0.8 ug CoraLite® Plus 488 Anti-Human LAMR1/RPSA (CL488-67324, Clone:2A9A1) (red), or 0.8 ug Isotype Control. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

经过测试的应用

Positive IF/ICC detected inA549 cells

Mouse monoclonal antibodies of IgA isotype can be detected with "anti-mouse IgG (H+L)" secondary antibodies.

For other applications, we recommend the unconjugated version of this antibody, 67324-1-Ig

推荐稀释比

ApplicationDilution
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL488-67324 targets LAMR1/RPSA in IF/ICC applications and shows reactivity with human, mouse, rat samples.

Tested Applications IF/ICC Application Description
Tested Reactivity human, mouse, rat
Immunogen LAMR1/RPSA fusion protein Ag6190 种属同源性预测
Host / Isotype Mouse / IgM
Class Monoclonal
Type Antibody
Full Name ribosomal protein SA
Synonyms LAMR1,RPSA, RPSA, 67LR, 67 kDa laminin receptor, 40S ribosomal protein SA
Calculated Molecular Weight 33 kDa
Observed Molecular Weight 37-40 kDa
GenBank Accession NumberBC050688
Gene Symbol RPSA
Gene ID (NCBI) 3921
Conjugate CoraLite® Plus 488 Fluorescent Dye
Excitation/Emission Maxima Wavelengths493 nm / 522 nm
Form Liquid
Purification MethodThiophilic affinity chromatograph
UNIPROT IDP08865
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

The ribosomal protein SA (RPSA), previously named 67 kD laminin receptor(67LR), 37 kD laminin receptor precursor (37LRP) and p40 ribosome-associated protein, is a multifunctional protein, that plays a role in a number of pathological processes, such as cancer and prion diseases. It is overexpressed in various cancer cell lines, and the the level of the laminin receptor transcript is higher in colon carcinoma tissue and lung cancer cell line than their normal counterparts.

实验方案

Product Specific Protocols
IF protocol for CL Plus 488 LAMR1/RPSA antibody CL488-67324Download protocol
Standard Protocols
Click here to view our Standard Protocols