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验证数据展示

  • WB analysis using 67810-1-Ig

    WB analysis using 67810-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 67810-1-Ig (LARP1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  • IHC staining of human ovary tumor using 67810-1-Ig

    IHC staining of human ovary tumor using 67810-1-Ig

    Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using 67810-1-Ig (LARP1 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with LARP1 antibody included
  • IF Staining of HepG2 using 67810-1-Ig

    IF Staining of HepG2 using 67810-1-Ig

    Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using LARP1 antibody (67810-1-Ig, Clone: 2E9F2 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L). Blue (DAPI).

  • IF Staining of HepG2 using 67810-1-Ig

    IF Staining of HepG2 using 67810-1-Ig

    Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using LARP1 antibody (67810-1-Ig, Clone: 2E9F2 ) at dilution of 1:800 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L).

LARP1 Monoclonal antibody

LARP1 Monoclonal Antibody for WB, IHC, IF/ICC, ELISA
Cat No. 67810-1-Ig

产品说明书

CloneNo. 2E9F2

宿主/亚型

Mouse / IgG1

种属反应性

Human, mouse, rat

应用

WB, IHC, IF/ICC, ELISA

别名

KIAA0731, La related protein 1, LARP, LARP1

缓冲液配方:  PBS and Azide
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inHCT 116 cells, HSC-T6 cells, NIH/3T3 cells, HeLa cells, HepG2 cells, Jurkat cells, K-562 cells
Positive IHC detected inhuman ovary tumor tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHepG2 cells
Planning an IHC experiment? We recommend our IHCeasy LARP1 Ready-To-Use IHC Kit. LARP1 primary antibody included.
Planning an IF experiment? We recommend our CoraLite® Plus 488, CoraLite®555 and CoraLite® Plus 647 conjugated versions of this antibody.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:2000-1:10000
Immunohistochemistry (IHC)IHC : 1:500-1:2000
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

67810-1-Ig targets LARP1 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with Human, mouse, rat samples.

经测试应用 WB, IHC, IF/ICC, ELISA Application Description
经测试反应性 Human, mouse, rat
免疫原 LARP1 fusion protein Ag29993 种属同源性预测
宿主/亚型 Mouse / IgG1
抗体类别 Monoclonal
产品类型 Antibody
全称 La ribonucleoprotein domain family, member 1
别名 KIAA0731, La related protein 1, LARP, LARP1
计算分子量 1096 aa, 124 kDa
观测分子量140-150 kDa
GenBank蛋白编号BC033856
基因名称 LARP1
Gene ID (NCBI) 23367
RRIDAB_2918573
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein G purification
UNIPROT IDQ6PKG0
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for LARP1 antibody 67810-1-IgDownload protocol
IHC protocol for LARP1 antibody 67810-1-IgDownload protocol
IF protocol for LARP1 antibody 67810-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols