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CoraLite®555-conjugated LARP1 Monoclonal antibody

LARP1 Monoclonal Antibody for IF/ICC, FC (Intra)

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse, rat

Applications

IF/ICC, FC (Intra)

Conjugate

CoraLite®555 Fluorescent Dye

CloneNo.

2E9F2

Cat no : CL555-67810

Print datasheet

Synonyms

LARP, La-related protein 1, La related protein 1, KIAA0731, 2E9F2

验证数据展示

  • IF Staining of HepG2 using CL555-67810

    IF Staining of HepG2 using CL555-67810

    Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using CoraLite®555 LARP1 antibody (CL555-67810, Clone: 2E9F2 ) at dilution of 1:400.

  • FC experiment of HepG2 using CL555-67810

    FC experiment of HepG2 using CL555-67810

    1X10^6 HepG2 cells were intracellularly stained with 0.4 ug CoraLite®555 Anti-Human LARP1 (CL555-67810, Clone:2E9F2) (red), or 0.4 ug Control Antibody. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

经过测试的应用

Positive IF/ICC detected inHepG2 cells
Positive FC (Intra) detected inHepG2 cells
Planning an IHC experiment? We recommend our IHCeasy LARP1 Ready-To-Use IHC Kit. LARP1 primary antibody included.
For other applications, we recommend the unconjugated version of this antibody, 67810-1-Ig

推荐稀释比

ApplicationDilution
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL555-67810 targets LARP1 in IF/ICC, FC (Intra) applications and shows reactivity with human, mouse, rat samples.

Tested Applications IF/ICC, FC (Intra) Application Description
Tested Reactivity human, mouse, rat
Immunogen LARP1 fusion protein Ag29993 种属同源性预测
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Full Name La ribonucleoprotein domain family, member 1
Synonyms LARP, La-related protein 1, La related protein 1, KIAA0731, 2E9F2
Calculated Molecular Weight 1096 aa, 124 kDa
Observed Molecular Weight140-150 kDa
GenBank Accession NumberBC033856
Gene Symbol LARP1
Gene ID (NCBI) 23367
RRIDAB_2919705
Conjugate CoraLite®555 Fluorescent Dye
Excitation/Emission Maxima Wavelengths557 nm / 570 nm
Form Liquid
Purification MethodProtein G purification
UNIPROT IDQ6PKG0
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

LARP1, full named as La-related Protein 1, is an oncogenic RNA-binding protein required for ribosome biogenesis and cancer cell survival. (PMID: 32286153)It's has been proposed to function downstream of mTORC1 to regulate the translation of 5'TOP mRNAs such as those encoding ribosome proteins (RP). (PMID: 28650797) LARP1 has 2 isoforms with MW of 123-150KDa. With CL647, immunoflorecence detected location in cytoplasm.

实验方案

Product Specific Protocols
IF protocol for CL555 LARP1 antibody CL555-67810Download protocol
FC protocol for CL555 LARP1 antibody CL555-67810Download protocol
Standard Protocols
Click here to view our Standard Protocols