验证数据展示
经过测试的应用
| Positive IF/ICC detected in | HepG2 cells |
Planning an IHC experiment? We recommend our IHCeasy LARP1 Ready-To-Use IHC Kit. LARP1 primary antibody included.
For other applications, we recommend the unconjugated version of this antibody, 13708-1-AP
推荐稀释比
| Application | Dilution |
|---|---|
| Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
CL488-13708 targets LARP1 in IF/ICC applications and shows reactivity with human, mouse, rat, drosophila samples.
| Tested Applications | IF/ICC Application Description |
| Tested Reactivity | human, mouse, rat, drosophila |
| Immunogen | LARP1 fusion protein Ag4645 种属同源性预测 |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Full Name | La ribonucleoprotein domain family, member 1 |
| Synonyms | KIAA0731, La related protein 1, LARP, LARP1 |
| Calculated Molecular Weight | 1096 aa, 124 kDa |
| Observed Molecular Weight | 140-150 kDa |
| GenBank Accession Number | BC033856 |
| Gene Symbol | LARP1 |
| Gene ID (NCBI) | 23367 |
| RRID | AB_3083946 |
| Conjugate | CoraLite® Plus 488 Fluorescent Dye |
| Excitation/Emission Maxima Wavelengths | 493 nm / 522 nm |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q6PKG0 |
| Storage Buffer | PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3. |
| Storage Conditions | Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
LARP1, full named as La-related Protein 1, is an oncogenic RNA-binding protein required for ribosome biogenesis and cancer cell survival. (PMID: 32286153)It's has been proposed to function downstream of mTORC1 to regulate the translation of 5'TOP mRNAs such as those encoding ribosome proteins (RP). (PMID: 28650797) LARP1 has 2 isoforms with MW of 123-150KDa. With CL647, immunoflorecence detected location in cytoplasm.
实验方案
| Product Specific Protocols | |
|---|---|
| IF protocol for CL Plus 488 LARP1 antibody CL488-13708 | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |