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LARP2 Recombinant antibody

LARP2 Uni-rAbTM Recombinant Antibody for IHC, IF/ICC, FC (Intra), ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

IHC, IF/ICC, FC (Intra), ELISA

Conjugate

Unconjugated

CloneNo.

242486E9

Cat no : 84898-3-RR

Print datasheet

Synonyms

LARP1B, La-related protein 2, La-related protein 1B, La related protein 2, La related protein 1B

验证数据展示

  • IHC staining of mouse testis using 84898-3-RR

    IHC staining of mouse testis using 84898-3-RR

    Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 84898-3-RR (LARP2 antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IF Staining of U2OS using 84898-3-RR

    IF Staining of U2OS using 84898-3-RR

    Immunofluorescent analysis of (4% PFA) fixed U2OS cells using LARP2 antibody (84898-3-RR, Clone: 242486E9 ) at dilution of 1:500 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red).

  • FC experiment of HeLa using 84898-3-RR

    FC experiment of HeLa using 84898-3-RR

    1x10^6 HeLa cells were intracellularly stained with 0.25 ug LARP2 Recombinant antibody (84898-3-RR, Clone:242486E9) and APC-Conjugated Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Rabbit IgG Isotype Control Recombinant Antibody (98136-1-RR, Clone: 240953C9) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

  • Affinity and Kinetic Characterization of 84898-3-RR

    Affinity and Kinetic Characterization of 84898-3-RR

    Biolayer interferometry (BLl) kinetic assays of 84898-3-RR against Human LARP2 were performed. The affinity constant is below 1 pM.

经过测试的应用

Positive IHC detected inmouse testis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inU2OS cells
Positive FC (Intra) detected inHeLa cells

推荐稀释比

ApplicationDilution
Immunohistochemistry (IHC)IHC : 1:200-1:800
Immunofluorescence (IF)/ICCIF/ICC : 1:250-1:1000
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

84898-3-RR targets LARP2 in IHC, IF/ICC, FC (Intra), ELISA applications and shows reactivity with human samples.

Tested Applications IHC, IF/ICC, FC (Intra), ELISA Application Description
Tested Reactivity human
Immunogen LARP2 fusion protein Ag11043 种属同源性预测
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Full Name La ribonucleoprotein domain family, member 2
Synonyms LARP1B, La-related protein 2, La-related protein 1B, La related protein 2, La related protein 1B
Calculated Molecular Weight 201 aa, 24 kDa
GenBank Accession NumberBC030516
Gene Symbol LARP2
Gene ID (NCBI) 55132
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDQ659C4
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
IHC protocol for LARP2 antibody 84898-3-RRDownload protocol
IF protocol for LARP2 antibody 84898-3-RRDownload protocol
FC protocol for LARP2 antibody 84898-3-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols