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LAT Recombinant antibody, PBS Only

LAT Uni-rAbTM Recombinant Antibody for WB, IF/ICC, FC (Intra), Indirect ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IF/ICC, FC (Intra), Indirect ELISA

Conjugate

Unconjugated

CloneNo.

2P13

Cat no : 82901-1-PBS

Print datasheet

Synonyms

p36-38, p36 38, Linker for activation of T-cells family member 1, LAT1, 36 kDa phosphotyrosine adapter protein

验证数据展示

  • WB analysis using 82901-1-RR (same clone as 82901-1-PBS)

    WB analysis using 82901-1-RR (same clone as 82901-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 82901-1-RR (LAT antibody) at dilution of 1:2500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 82901-1-PBS in a different storage buffer formulation.

  • IF Staining of Jurkat using 82901-1-RR (same clone as 82901-1-PBS)

    IF Staining of Jurkat using 82901-1-RR (same clone as 82901-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed Jurkat cells using LAT antibody (82901-1-RR, Clone: 2P13 ) at dilution of 1:1500 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 82901-1-PBS in a different storage buffer formulation.

  • FC experiment of Jurkat using 82901-1-RR (same clone as 82901-1-PBS)

    FC experiment of Jurkat using 82901-1-RR (same clone as 82901-1-PBS)

    1x10^6 Jurkat cells were intracellularly stained with 0.25 ug LAT Recombinant antibody (82901-1-RR, Clone:2P13) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.25 ug Isotype Control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 82901-1-PBS in a different storage buffer formulation.

产品信息

82901-1-PBS targets LAT in WB, IF/ICC, FC (Intra), Indirect ELISA applications and shows reactivity with human, mouse samples.

Tested Applications WB, IF/ICC, FC (Intra), Indirect ELISA Application Description
Tested Reactivity human, mouse
Immunogen Peptide 种属同源性预测
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Full Name linker for activation of T cells
Synonyms p36-38, p36 38, Linker for activation of T-cells family member 1, LAT1, 36 kDa phosphotyrosine adapter protein
Calculated Molecular Weight 262 aa, 28 kDa
Observed Molecular Weight 36-38 kDa
GenBank Accession NumberBC011563
Gene Symbol LAT
Gene ID (NCBI) 27040
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDO43561
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C