• All
  • Primary Antibodies
  • Conjugated Antibodies for IF
  • Conjugated Antibodies for FC
  • Secondary Antibodies
  • Antibody Labeling KitsNew
  • ELISA Kits
  • IHC Kits
  • Magnetic Cell Separation Kits
  • Cytokines & Growth Factors
  • Neutralizing/activating Antibodies
  • Nanobody-based Reagents
  • Accessory Products and Kits
  • Fusion Proteins
×
×
Host
0
Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
0
Compatible Species
0
Conjugate
0

验证数据展示

  • WB analysis using 82901-1-RR (same clone as 82901-1-PBS)

    WB analysis using 82901-1-RR (same clone as 82901-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 82901-1-RR (LAT antibody) at dilution of 1:2500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 82901-1-PBS in a different storage buffer formulation.

  • IF Staining of Jurkat using 82901-1-RR (same clone as 82901-1-PBS)

    IF Staining of Jurkat using 82901-1-RR (same clone as 82901-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed Jurkat cells using LAT antibody (82901-1-RR, Clone: 2P13 ) at dilution of 1:1500 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 82901-1-PBS in a different storage buffer formulation.

  • WB analysis of Jurkat using 82901-1-RR (same clone as 82901-1-PBS)

    WB analysis of Jurkat using 82901-1-RR (same clone as 82901-1-PBS)

    WB result of LAT antibody (82901-1-RR; 1:400; incubated at room temperature for 1.5 hours) with sh-Control and sh-LAT transfected Jurkat cells. This data was developed using the same antibody clone with 82901-1-PBS in a different storage buffer formulation.

  • FC experiment of Jurkat using 82901-1-RR (same clone as 82901-1-PBS)

    FC experiment of Jurkat using 82901-1-RR (same clone as 82901-1-PBS)

    1x10^6 Jurkat cells were intracellularly stained with 0.25 ug LAT Recombinant antibody (82901-1-RR, Clone:2P13) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.25 ug Isotype Control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 82901-1-PBS in a different storage buffer formulation.

  • Featured Product
  • KD/KO验证

LAT Recombinant antibody, PBS Only

LAT Uni-rAbTM Recombinant Antibody for WB, IF/ICC, FC (Intra), Indirect ELISA
Cat No. 82901-1-PBS

产品说明书

CloneNo. 2P13

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse

应用

WB, IF/ICC, FC (Intra), Indirect ELISA

别名

p36-38, p36 38, Linker for activation of T-cells family member 1, LAT1, 36 kDa phosphotyrosine adapter protein

缓冲液配方:  PBS Only
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格: 

-/ -

点击咨询售前客服

点击咨询售后客服

产品信息

82901-1-PBS targets LAT in WB, IF/ICC, FC (Intra), Indirect ELISA applications and shows reactivity with human, mouse samples.

经测试应用 WB, IF/ICC, FC (Intra), Indirect ELISA Application Description
经测试反应性 human, mouse
免疫原 Peptide 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 linker for activation of T cells
别名 p36-38, p36 38, Linker for activation of T-cells family member 1, LAT1, 36 kDa phosphotyrosine adapter protein
计算分子量 262 aa, 28 kDa
观测分子量 36-38 kDa
GenBank蛋白编号BC011563
基因名称 LAT
Gene ID (NCBI) 27040
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purification
UNIPROT IDO43561
储存缓冲液 PBS only , pH 7.3
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

LAT is a membrane-localized adaptor protein which is primarily concentrated in microdomains by palmitoylation. LAT plays a central role in T-cell activation by nucleating signaling complexes that are critical for the propagation of T-cell signals from the plasma membrane to the cellular interior. In addition to being a positive regulator of T cell signaling, LAT also recruits several negative regulatory proteins, including kinases, phosphatases, and ubiquitin ligases, which ultimately leads to signal termination. LAT is subject to ubiquitylation, and ubiquitin-resistant mutants of LAT display enhanced signaling.