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LAT Recombinant antibody

LAT Uni-rAbTM Recombinant Antibody for WB, IF/ICC, FC (Intra), ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IF/ICC, FC (Intra), ELISA

Conjugate

Unconjugated

CloneNo.

2P13

Cat no : 82901-1-RR

Print datasheet

Synonyms

p36-38, p36 38, Linker for activation of T-cells family member 1, LAT1, 36 kDa phosphotyrosine adapter protein

验证数据展示

  • WB analysis using 82901-1-RR

    WB analysis using 82901-1-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 82901-1-RR (LAT antibody) at dilution of 1:2500 incubated at room temperature for 1.5 hours.

  • IF Staining of Jurkat using 82901-1-RR

    IF Staining of Jurkat using 82901-1-RR

    Immunofluorescent analysis of (4% PFA) fixed Jurkat cells using LAT antibody (82901-1-RR, Clone: 2P13 ) at dilution of 1:1500 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

  • FC experiment of Jurkat using 82901-1-RR

    FC experiment of Jurkat using 82901-1-RR

    1x10^6 Jurkat cells were intracellularly stained with 0.25 ug LAT Recombinant antibody (82901-1-RR, Clone:2P13) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.25 ug Isotype Control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

经过测试的应用

Positive WB detected inJurkat cells, EL-4 cells, human platelets
Positive IF/ICC detected inJurkat cells
Positive FC (Intra) detected inJurkat cells

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:5000
Immunofluorescence (IF)/ICCIF/ICC : 1:750-1:3000
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

82901-1-RR targets LAT in WB, IF/ICC, FC (Intra), ELISA applications and shows reactivity with human, mouse samples.

Tested Applications WB, IF/ICC, FC (Intra), ELISA Application Description
Tested Reactivity human, mouse
Immunogen Peptide 种属同源性预测
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Full Name linker for activation of T cells
Synonyms p36-38, p36 38, Linker for activation of T-cells family member 1, LAT1, 36 kDa phosphotyrosine adapter protein
Calculated Molecular Weight 262 aa, 28 kDa
Observed Molecular Weight 36-38 kDa
GenBank Accession NumberBC011563
Gene Symbol LAT
Gene ID (NCBI) 27040
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDO43561
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

LAT is a membrane-localized adaptor protein which is primarily concentrated in microdomains by palmitoylation. LAT plays a central role in T-cell activation by nucleating signaling complexes that are critical for the propagation of T-cell signals from the plasma membrane to the cellular interior. In addition to being a positive regulator of T cell signaling, LAT also recruits several negative regulatory proteins, including kinases, phosphatases, and ubiquitin ligases, which ultimately leads to signal termination. LAT is subject to ubiquitylation, and ubiquitin-resistant mutants of LAT display enhanced signaling.

实验方案

Product Specific Protocols
WB protocol for LAT antibody 82901-1-RRDownload protocol
IF protocol for LAT antibody 82901-1-RRDownload protocol
FC protocol for LAT antibody 82901-1-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols