CoraLite® Plus 488-conjugated LAT Recombinant antibody
LAT Uni-rAbTM Recombinant Antibody for IF/ICC, FC (Intra)
Host / Isotype
Rabbit / IgG
Reactivity
human, mouse
Applications
IF/ICC, FC (Intra)
Conjugate
CoraLite® Plus 488 Fluorescent Dye
CloneNo.
2P13
验证数据展示
经过测试的应用
| Positive IF/ICC detected in | Jurkat cells |
| Positive FC (Intra) detected in | Jurkat cells |
For other applications, we recommend the unconjugated version of this antibody, 82901-1-PBS
推荐稀释比
| Application | Dilution |
|---|---|
| Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
| Flow Cytometry (FC) (INTRA) | FC (INTRA) : 0.80 ug per 10^6 cells in a 100 µl suspension |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
CL488-82901 targets LAT in IF/ICC, FC (Intra) applications and shows reactivity with human, mouse samples.
| Tested Applications | IF/ICC, FC (Intra) Application Description |
| Tested Reactivity | human, mouse |
| Immunogen | Peptide 种属同源性预测 |
| Host / Isotype | Rabbit / IgG |
| Class | Recombinant |
| Type | Antibody |
| Full Name | linker for activation of T cells |
| Synonyms | p36-38, p36 38, Linker for activation of T-cells family member 1, LAT1, 36 kDa phosphotyrosine adapter protein |
| Calculated Molecular Weight | 262 aa, 28 kDa |
| Observed Molecular Weight | 36-38 kDa |
| GenBank Accession Number | BC011563 |
| Gene Symbol | LAT |
| Gene ID (NCBI) | 27040 |
| Conjugate | CoraLite® Plus 488 Fluorescent Dye |
| Excitation/Emission Maxima Wavelengths | 493 nm / 522 nm |
| Form | Liquid |
| Purification Method | Protein A purification |
| UNIPROT ID | O43561 |
| Storage Buffer | PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3. |
| Storage Conditions | Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
LAT is a membrane-localized adaptor protein which is primarily concentrated in microdomains by palmitoylation. LAT plays a central role in T-cell activation by nucleating signaling complexes that are critical for the propagation of T-cell signals from the plasma membrane to the cellular interior. In addition to being a positive regulator of T cell signaling, LAT also recruits several negative regulatory proteins, including kinases, phosphatases, and ubiquitin ligases, which ultimately leads to signal termination. LAT is subject to ubiquitylation, and ubiquitin-resistant mutants of LAT display enhanced signaling.
实验方案
| Product Specific Protocols | |
|---|---|
| IF protocol for CL Plus 488 LAT antibody CL488-82901 | Download protocol |
| FC protocol for CL Plus 488 LAT antibody CL488-82901 | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |