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验证数据展示

  • IF Staining of HeLa using CL488-17049

    IF Staining of HeLa using CL488-17049

    Immunofluorescent analysis of (-20°C Methanol) fixed HeLa cells using CoraLite® Plus 488 LATS1 antibody (CL488-17049) at dilution of 1:200.

  • Featured Product
  • KD/KO验证

CoraLite® Plus 488-conjugated LATS1 Polyclonal antibody

LATS1 Polyclonal Antibody for IF/ICC
Cat No. CL488-17049

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

IF/ICC

别名

h warts, LATS1, WARTS, WARTS protein kinase, wts

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  CoraLite® Plus 488
规格: 

-/ -

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经过测试的应用

Positive IF/ICC detected inHeLa cells
For other applications, we recommend the unconjugated version of this antibody, 17049-1-AP

推荐稀释比

应用推荐稀释比
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL488-17049 targets LATS1 in IF/ICC applications and shows reactivity with human samples.

经测试应用 IF/ICC Application Description
经测试反应性 human
免疫原 LATS1 fusion protein Ag10709 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 LATS, large tumor suppressor, homolog 1 (Drosophila)
别名 h warts, LATS1, WARTS, WARTS protein kinase, wts
计算分子量 15 kDa, 127 kDa
观测分子量 140-150 kDa, 120 kDa
GenBank蛋白编号BC015665
基因名称 LATS1
Gene ID (NCBI) 9113
RRIDAB_3083996
偶联类型 CoraLite® Plus 488 Fluorescent Dye
最大激发/发射波长493 nm / 522 nm
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDO95835
储存缓冲液 PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA , pH 7.3
储存条件Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

LATS1(Large tumor suppressor homolog 1) is also named as WARTS and belongs to the AGC Ser/Thr protein kinase family. The gene encodes a highly conserved (from fly to human) protein kinase that plays a crucial role in the prevention of tumor formation by controlling the progression of mitosis. The expression of both long(170 kDa) and short lats1 isoforms(120 kDa) in vertebrate retinal cells raises the possibility that these lats1 proteins may act as negative key regulators of the cell cycle each of them performing a unique role (PMID:15777619). In mammalian cells, LATS1 was phosphorylated in a cell cycle-dependent manner and complexed with CDC2 in early mitosis (PMID:9988268). LATS1 also can be detected as 120 kDa and 140-150 kDa, and play a key role in the regulation of Hippo pathway (PMID: 27940445).

实验方案

Product Specific Protocols
IF protocol for CL Plus 488 LATS1 antibody CL488-17049Download protocol
Standard Protocols
Click here to view our Standard Protocols