LBR Monoclonal antibody

LBR Monoclonal Antibody for WB, IHC, IF/ICC, ELISA
Cat No. 68378-1-Ig

产品说明书

CloneNo. 1A1B7

宿主/亚型

Mouse / IgG1

种属反应性

human, mouse, rat

应用

WB, IHC, IF/ICC, ELISA

Delta(14)-sterol reductase LBR, C14SR, C-14 sterol reductase, 3-beta-hydroxysterol Delta, 1A1B7

缓冲液配方:  PBS and Azide
PBS and Azide
PBS Only
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -


经过测试的应用

Positive WB detected inU2OS cells, HSC-T6 cells, LNCaP cells, HeLa cells, HEK-293 cells, Jurkat cells, K-562 cells, ROS1728 cells
Positive IHC detected inmouse testis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHeLa cells
Planning an IHC experiment? We recommend our IHCeasy LBR Ready-To-Use IHC Kit. LBR primary antibody included.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:5000-1:50000
Immunohistochemistry (IHC)IHC : 1:500-1:2000
Immunofluorescence (IF)/ICCIF/ICC : 1:500-1:2000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

68378-1-Ig targets LBR in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, IF/ICC, ELISA Application Description
经测试反应性 human, mouse, rat
免疫原 LBR fusion protein Ag25601 种属同源性预测
宿主/亚型 Mouse / IgG1
抗体类别 Monoclonal
产品类型 Antibody
全称 lamin B receptor
别名 Delta(14)-sterol reductase LBR, C14SR, C-14 sterol reductase, 3-beta-hydroxysterol Delta, 1A1B7
计算分子量 615 aa, 71 kDa
观测分子量58 kDa
GenBank蛋白编号BC020079
基因名称 LBR
Gene ID (NCBI) 3930
RRIDAB_3085096
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purification
UNIPROT IDQ14739
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Lamin-B receptor (LBR) is an integral membrane protein of the inner nuclear membrane that contains a hydrophilic N-terminal end protruding into the nucleoplasm, eight hydrophobic segments that span the membrane and a short, nucleoplasmic C-terminal tail (PMID: 28858257). LBR anchors the lamina and the heterochromatin to the inner nuclear membrane (PMID: 10828963; 28858257). It is also essential for cholesterol synthesis (PMID: 27336722). Mutations of the LBR gene have been associated with autosomal recessive HEM/Greenberg skeletal dysplasia and Pelger-Huët anomaly and Greenberg skeletal dysplasia (PMID: 12618959; 12490533). The calculated molecular mass of LBR is 71 kDa, which is larger than the apparent molecular mass of 58 kDa, probably due to the aberrant migration of membrane proteins subjected to SDS-PAGE (PMID: 2847165; 2170422).

实验方案

Product Specific Protocols
WB protocol for LBR antibody 68378-1-IgDownload protocol
IHC protocol for LBR antibody 68378-1-IgDownload protocol
IF protocol for LBR antibody 68378-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols
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