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LBR Monoclonal antibody, PBS Only

LBR Monoclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

1A1B7

Cat no : 68378-1-PBS

Print datasheet

Synonyms

Delta(14)-sterol reductase LBR, C14SR, C-14 sterol reductase, 3-beta-hydroxysterol Delta, 1A1B7

验证数据展示

  • WB analysis using 68378-1-Ig (same clone as 68378-1-PBS)

    WB analysis using 68378-1-Ig (same clone as 68378-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 68378-1-Ig (LBR antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 68378-1-PBS in a different storage buffer formulation.

  • IHC staining of mouse testis using 68378-1-Ig (same clone as 68378-1-PBS)

    IHC staining of mouse testis using 68378-1-Ig (same clone as 68378-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 68378-1-Ig (LBR antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 68378-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with LBR antibody included
  • IHC staining of mouse testis using 68378-1-Ig (same clone as 68378-1-PBS)

    IHC staining of mouse testis using 68378-1-Ig (same clone as 68378-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 68378-1-Ig (LBR antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 68378-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with LBR antibody included
  • IF Staining of HeLa using 68378-1-Ig (same clone as 68378-1-PBS)

    IF Staining of HeLa using 68378-1-Ig (same clone as 68378-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using LBR antibody (68378-1-Ig, Clone: 1A1B7 ) at dilution of 1:1000 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) (SA00013-1). This data was developed using the same antibody clone with 68378-1-PBS in a different storage buffer formulation.

Planning an IHC experiment? We recommend our IHCeasy LBR Ready-To-Use IHC Kit. LBR primary antibody included.

产品信息

68378-1-PBS targets LBR in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications WB, IHC, IF/ICC, Indirect ELISA Application Description
Tested Reactivity human, mouse, rat
Immunogen LBR fusion protein Ag25601 种属同源性预测
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Full Name lamin B receptor
Synonyms Delta(14)-sterol reductase LBR, C14SR, C-14 sterol reductase, 3-beta-hydroxysterol Delta, 1A1B7
Calculated Molecular Weight 615 aa, 71 kDa
Observed Molecular Weight58 kDa
GenBank Accession NumberBC020079
Gene Symbol LBR
Gene ID (NCBI) 3930
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDQ14739
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

Lamin-B receptor (LBR) is an integral membrane protein of the inner nuclear membrane that contains a hydrophilic N-terminal end protruding into the nucleoplasm, eight hydrophobic segments that span the membrane and a short, nucleoplasmic C-terminal tail (PMID: 28858257). LBR anchors the lamina and the heterochromatin to the inner nuclear membrane (PMID: 10828963; 28858257). It is also essential for cholesterol synthesis (PMID: 27336722). Mutations of the LBR gene have been associated with autosomal recessive HEM/Greenberg skeletal dysplasia and Pelger-Huët anomaly and Greenberg skeletal dysplasia (PMID: 12618959; 12490533). The calculated molecular mass of LBR is 71 kDa, which is larger than the apparent molecular mass of 58 kDa, probably due to the aberrant migration of membrane proteins subjected to SDS-PAGE (PMID: 2847165; 2170422).