验证数据展示
经过测试的应用
| Positive IF/ICC detected in | Chloroquine treated HepG2 cells |
Planning an IHC experiment? We recommend our IHCeasy LC3 Ready-To-Use IHC Kit. LC3 primary antibody included.
For other applications, we recommend the unconjugated version of this antibody, 81004-1-PBS
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Immunofluorescence (IF)/ICC | IF/ICC : 1:250-1:1000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
CL488-81004 targets LC3 in IF/ICC applications and shows reactivity with Human, Mouse, Rat, Pig samples.
| 经测试应用 | IF/ICC Application Description |
| 经测试反应性 | Human, Mouse, Rat, Pig |
| 免疫原 | LC3 fusion protein Ag6144 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Recombinant |
| 产品类型 | Antibody |
| 全称 | microtubule-associated protein 1 light chain 3 beta |
| 别名 | LC3B, MAP1A/1BLC3, MAP1A/MAP1B LC3 B, MAP1A/MAP1B light chain 3 B, MAP1ALC3 |
| 计算分子量 | 15 kDa |
| 观测分子量 | 14-18 kDa |
| GenBank蛋白编号 | BC067797 |
| 基因名称 | LC3B |
| Gene ID (NCBI) | 81631 |
| ENSEMBL Gene ID | ENSG00000140941 |
| RRID | AB_3084496 |
| 偶联类型 | CoraLite® Plus 488 Fluorescent Dye |
| 最大激发/发射波长 | 493 nm / 522 nm |
| 形式 | Liquid |
| 纯化方式 | Protein A purification |
| UNIPROT ID | Q9GZQ8 |
| 储存缓冲液 | PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3. |
| 储存条件 | Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
Map1LC3, also known as LC3, is the human homolog of yeast Atg8 and is involved in the formation of autophagosomal vacuoles, called autophagosomes. Three human Map1LC3 isoforms, MAP1LC3A, MAP1LC3B, and MAP1LC3C, undergo post-translational modifications during autophagy. And they differ in their post-translation modifications during autophagy. Map1LC3 also exists in two modified forms, an 18 kDa cytoplasmic form that was originally identified as a subunit of the microtubule-associated protein 1, and a 14-16 kDa form that is associated with the autophagosome membrane.
实验方案
| Product Specific Protocols | |
|---|---|
| IF protocol for CL Plus 488 LC3 antibody CL488-81004 | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |