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- KD/KO Validated
CoraLite® Plus 488-conjugated LC3 Recombinant antibody
LC3 Uni-rAbTM Recombinant Antibody for IF/ICC
Host / Isotype
Rabbit / IgG
Reactivity
Human, Mouse, Rat, Pig
Applications
IF/ICC
Conjugate
CoraLite® Plus 488 Fluorescent Dye
CloneNo.
5P12
验证数据展示
经过测试的应用
| Positive IF/ICC detected in | Chloroquine treated HepG2 cells |
Planning an IHC experiment? We recommend our IHCeasy LC3 Ready-To-Use IHC Kit. LC3 primary antibody included.
For other applications, we recommend the unconjugated version of this antibody, 81004-1-PBS
推荐稀释比
| Application | Dilution |
|---|---|
| Immunofluorescence (IF)/ICC | IF/ICC : 1:250-1:1000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
CL488-81004 targets LC3 in IF/ICC applications and shows reactivity with Human, Mouse, Rat, Pig samples.
| Tested Applications | IF/ICC Application Description |
| Tested Reactivity | Human, Mouse, Rat, Pig |
| Immunogen | LC3 fusion protein Ag6144 种属同源性预测 |
| Host / Isotype | Rabbit / IgG |
| Class | Recombinant |
| Type | Antibody |
| Full Name | microtubule-associated protein 1 light chain 3 beta |
| Synonyms | LC3B, MAP1A/1BLC3, MAP1A/MAP1B LC3 B, MAP1A/MAP1B light chain 3 B, MAP1ALC3 |
| Calculated Molecular Weight | 15 kDa |
| Observed Molecular Weight | 14-18 kDa |
| GenBank Accession Number | BC067797 |
| Gene Symbol | LC3B |
| Gene ID (NCBI) | 81631 |
| ENSEMBL Gene ID | ENSG00000140941 |
| Conjugate | CoraLite® Plus 488 Fluorescent Dye |
| Excitation/Emission Maxima Wavelengths | 493 nm / 522 nm |
| Form | Liquid |
| Purification Method | Protein A purification |
| UNIPROT ID | Q9GZQ8 |
| Storage Buffer | PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3. |
| Storage Conditions | Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
Map1LC3, also known as LC3, is the human homolog of yeast Atg8 and is involved in the formation of autophagosomal vacuoles, called autophagosomes. Three human Map1LC3 isoforms, MAP1LC3A, MAP1LC3B, and MAP1LC3C, undergo post-translational modifications during autophagy. And they differ in their post-translation modifications during autophagy. Map1LC3 also exists in two modified forms, an 18 kDa cytoplasmic form that was originally identified as a subunit of the microtubule-associated protein 1, and a 14-16 kDa form that is associated with the autophagosome membrane.
实验方案
| Product Specific Protocols | |
|---|---|
| IF protocol for CL Plus 488 LC3 antibody CL488-81004 | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |