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LC3B-Specific Polyclonal antibody, PBS Only

LC3B-Specific Polyclonal Antibody for WB, IHC, IF/ICC, FC (Intra), Indirect ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, FC (Intra), Indirect ELISA

Conjugate

Unconjugated

Cat no : 18725-1-PBS

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Synonyms

验证数据展示

  • WB analysis of human brain using 18725-1-AP (same clone as 18725-1-PBS)

    WB analysis of human brain using 18725-1-AP (same clone as 18725-1-PBS)

    human brain tissue were subjected to SDS PAGE followed by western blot with 18725-1-AP (LC3B-Specific antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 18725-1-PBS in a different storage buffer formulation.

  • IHC staining of mouse brain using 18725-1-AP (same clone as 18725-1-PBS)

    IHC staining of mouse brain using 18725-1-AP (same clone as 18725-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 18725-1-AP (LC3B-Specific antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 18725-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with LC3 antibody included
  • FC experiment of HeLa using 18725-1-AP (same clone as 18725-1-PBS)

    FC experiment of HeLa using 18725-1-AP (same clone as 18725-1-PBS)

    1X10^6 HeLa cells were intracellularly stained with 0.4 ug Anti-Human LC3B-Specific (18725-1-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Control Antibody. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 18725-1-PBS in a different storage buffer formulation.

  • IF Staining of HeLa using 18725-1-AP (same clone as 18725-1-PBS)

    IF Staining of HeLa using 18725-1-AP (same clone as 18725-1-PBS)

    Immunofluorescent analysis of (-20°C Ethanol) fixed Chloroquine treated HeLa cells using LC3B-Specific antibody (18725-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red). This data was developed using the same antibody clone with 18725-1-PBS in a different storage buffer formulation.

Planning an IHC experiment? We recommend our IHCeasy LC3 Ready-To-Use IHC Kit. LC3 primary antibody included.

产品信息

18725-1-PBS targets LC3B-Specific in WB, IHC, IF/ICC, FC (Intra), Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications WB, IHC, IF/ICC, FC (Intra), Indirect ELISA Application Description
Tested Reactivity human, mouse, rat
Immunogen Peptide 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name microtubule-associated protein 1 light chain 3 beta
Synonyms
Calculated Molecular Weight 15 kDa
Observed Molecular Weight 15 kDa, 18 kDa
GenBank Accession NumberNM_022818
Gene Symbol LC3B
Gene ID (NCBI) 81631
ENSEMBL Gene IDENSG00000140941
RRIDAB_2137745
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ9GZQ8
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

LC3B, also named as MAP1LC3B, MAP1A/1BLC3, belongs to the MAP1 LC3 family. It is a subunit of neuronal microtubule-associated MAP1A and MAP1B proteins, which are involved in microtubule assembly and important for neurogenesis. In cell biology, autophagy, or autophagocytosis, is a catabolic process involving the degradation of a cell's own components through the lysosomalmachinery. It is a major mechanism by which a starving cell reallocates nutrients from unnecessary processes to more-essential processes. Two forms of LC3, called LC3-I (17-19kd) and -II(14-16kd), were produced post-translationally in various cells. LC3-I is cytosolic, whereas LC3-II is membrane bound. The precursor molecule is cleaved by APG4B/ATG4B to form the cytosolic form, LC3-I. This is activated by APG7L/ATG7, transferred to ATG3 and conjugated to phospholipid to form the membrane-bound form, LC3-II. The amount of LC3-II is correlated with the extent of autophagosome formation. LC3-II is the first mammalian protein identified that specifically associates with autophagosome membranes. MAP1LC3 has 3 isoforms MAP1LC3A, MAP1LC3B and MAP1LC3C. MAP1LC3A and MAP1LC3C are produced by the proteolytic cleavage after the conserved C-terminal Gly residue, like their rat counterpart, MAP1LC3B does not undergo C-terminal cleavage and exists in a single modified form. This antibody is specific to LC3B.