LC3B-Specific Polyclonal antibody
LC3B-Specific Polyclonal Antibody for WB, IHC, IF/ICC, FC (Intra), ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human, mouse, rat and More (3)
Applications
WB, IHC, IF/ICC, FC (Intra), ELISA and More (2)
Conjugate
Unconjugated
318
验证数据展示
经过测试的应用
Positive WB detected in | human brain tissue, MCF-7 cells, TN treated Hela, A549 cells, UV treated HEK-293, mouse brain tissue, HepG2 cells |
Positive IHC detected in | mouse brain tissue, rat brain tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Positive IF/ICC detected in | Chloroquine treated HeLa cells, Chloroquine treated HepG2 cells |
Positive FC (Intra) detected in | HeLa cells |
推荐稀释比
Application | Dilution |
---|---|
Western Blot (WB) | WB : 1:300-1:1000 |
Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
Flow Cytometry (FC) (INTRA) | FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
产品信息
18725-1-AP targets LC3B-Specific in WB, IHC, IF/ICC, FC (Intra), IP, ELISA applications and shows reactivity with human, mouse, rat samples.
Tested Applications | WB, IHC, IF/ICC, FC (Intra), ELISA Application Description |
Cited Applications | WB, IHC, IF, IP |
Tested Reactivity | human, mouse, rat |
Cited Reactivity | human, mouse, rat, pig, zebrafish, bovine |
Immunogen | Peptide 种属同源性预测 |
Host / Isotype | Rabbit / IgG |
Class | Polyclonal |
Type | Antibody |
Full Name | microtubule-associated protein 1 light chain 3 beta |
Synonyms | LC3, MAP1LC3B, MAP1A/1BLC3, MAP1 light chain 3-like protein 2, LC3B |
Calculated Molecular Weight | 15 kDa |
Observed Molecular Weight | 15 kDa, 18 kDa |
GenBank Accession Number | NM_022818 |
Gene Symbol | LC3B |
Gene ID (NCBI) | 81631 |
ENSEMBL Gene ID | ENSG00000140941 |
RRID | AB_2137745 |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Antigen affinity purification |
UNIPROT ID | Q9GZQ8 |
Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
实验方案
Product Specific Protocols | |
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WB protocol for LC3B-Specific antibody 18725-1-AP | Download protocol |
IHC protocol for LC3B-Specific antibody 18725-1-AP | Download protocol |
IF protocol for LC3B-Specific antibody 18725-1-AP | Download protocol |
FC protocol for LC3B-Specific antibody 18725-1-AP | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |
发表文章
Species | Application | Title |
---|---|---|
Am J Respir Crit Care Med Acid Sphingomyelinase Inhibition Attenuates Cell Death in Mechanically-Ventilated Newborn Rat Lung. | ||
Nat Microbiol Chloroquine ameliorates carbon tetrachloride-induced acute liver injury in mice via the concomitant inhibition of inflammation and induction of apoptosis | ||
ACS Nano Nanoenabled Disruption of Multiple Barriers in Antigen Cross-Presentation of Dendritic Cells via Calcium Interference for Enhanced Chemo-Immunotherapy. | ||
Autophagy TRIM59 regulates autophagy through modulating both the transcription and the ubiquitination of BECN1. | ||
Autophagy STYK1 promotes autophagy through enhancing the assembly of autophagy-specific class III phosphatidylinositol 3-kinase complex I. | ||
Mater Today Bio Carbon dots enhance extracellular matrix secretion for dentin-pulp complex regeneration through PI3K/Akt/mTOR pathway-mediated activation of autophagy. |