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验证数据展示

  • IHC staining of human tonsillitis using 60162-1-Ig (same clone as 60162-1-PBS)

    IHC staining of human tonsillitis using 60162-1-Ig (same clone as 60162-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 60162-1-Ig (LCK antibody) at dilution of 1:1000 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 60162-1-PBS in a different storage buffer formulation.

  • IHC staining of human tonsillitis using 60162-1-Ig (same clone as 60162-1-PBS)

    IHC staining of human tonsillitis using 60162-1-Ig (same clone as 60162-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 60162-1-Ig (LCK antibody) at dilution of 1:1000 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 60162-1-PBS in a different storage buffer formulation.

  • IF Staining of human tonsillitis using 60162-1-Ig (same clone as 60162-1-PBS)

    IF Staining of human tonsillitis using 60162-1-Ig (same clone as 60162-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed human tonsillitis tissue using LCK antibody (60162-1-Ig, Clone: 2D10C2 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 60162-1-PBS in a different storage buffer formulation.

  • IF Staining of human tonsillitis using 60162-1-Ig (same clone as 60162-1-PBS)

    IF Staining of human tonsillitis using 60162-1-Ig (same clone as 60162-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed human tonsillitis tissue using LCK antibody (60162-1-Ig, Clone: 2D10C2 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 60162-1-PBS in a different storage buffer formulation.

  • WB analysis of pig thymus using 60162-1-Ig (same clone as 60162-1-PBS)

    WB analysis of pig thymus using 60162-1-Ig (same clone as 60162-1-PBS)

    pig thymus tissue were subjected to SDS PAGE followed by western blot with 60162-1-Ig (LCK antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 60162-1-PBS in a different storage buffer formulation.

  • WB analysis using 60162-1-Ig (same clone as 60162-1-PBS)

    WB analysis using 60162-1-Ig (same clone as 60162-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 60162-1-Ig (LCK antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 60162-1-PBS in a different storage buffer formulation.

  • IF Staining of HepG2 using 60162-1-Ig (same clone as 60162-1-PBS)

    IF Staining of HepG2 using 60162-1-Ig (same clone as 60162-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using LCK antibody (60162-1-Ig, Clone: 2D10C2 ) at dilution of 1:800 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L), CL594-phalloidin (red). This data was developed using the same antibody clone with 60162-1-PBS in a different storage buffer formulation.

  • IF Staining of HepG2 using 60162-1-Ig (same clone as 60162-1-PBS)

    IF Staining of HepG2 using 60162-1-Ig (same clone as 60162-1-PBS)

    Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using LCK antibody (60162-1-Ig, Clone: 2D10C2 ) at dilution of 1:800 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L), CL594-phalloidin (red). This data was developed using the same antibody clone with 60162-1-PBS in a different storage buffer formulation.

  • FC experiment of HepG2 using 60162-1-Ig (same clone as 60162-1-PBS)

    FC experiment of HepG2 using 60162-1-Ig (same clone as 60162-1-PBS)

    1X10^6 HepG2 cells were intracellularly stained with 0.4 ug Anti-Human LCK (60162-1-Ig, Clone:2D10C2) and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Control Antibody. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 60162-1-PBS in a different storage buffer formulation.

  • Cytometric bead array standard curve of MP51238-1

    Cytometric bead array standard curve of MP51238-1

    Cytometric bead array standard curve of MP51238-1, LCK Monoclonal Matched Antibody Pair, PBS Only. Capture antibody: 60162-1-PBS. Detection antibody: 60162-3-PBS. Standard:Ag7694. Range: 0.098-25 ng/mL.

LCK Monoclonal antibody, PBS Only (Capture)

LCK Monoclonal Antibody for WB, IHC, IF/ICC, IF-P, FC (Intra), Cytometric bead array, Indirect ELISA
Cat No. 60162-1-PBS

产品说明书

CloneNo. 2D10C2

宿主/亚型

Mouse / IgG1

种属反应性

human, rat, pig

应用

WB, IHC, IF/ICC, IF-P, FC (Intra), Cytometric bead array, Indirect ELISA

别名

Lymphocyte cell-specific protein-tyrosine kinase, LSK, Leukocyte C-terminal Src kinase, Lck p56, EC:2.7.10.2

缓冲液配方:  PBS Only
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格: 

-/ -

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产品信息

60162-1-PBS targets LCK as part of a matched antibody pair:

MP51238-1: 60162-1-PBS capture and 60162-3-PBS detection (validated in Cytometric bead array)

Unconjugated mouse monoclonal antibody pair in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

经测试应用 WB, IHC, IF/ICC, IF-P, FC (Intra), Cytometric bead array, Indirect ELISA Application Description
经测试反应性 human, rat, pig
免疫原 LCK fusion protein Ag7694 种属同源性预测
宿主/亚型 Mouse / IgG1
抗体类别 Monoclonal
产品类型 Antibody
全称 lymphocyte-specific protein tyrosine kinase
别名 Lymphocyte cell-specific protein-tyrosine kinase, LSK, Leukocyte C-terminal Src kinase, Lck p56, EC:2.7.10.2
计算分子量 539 aa, 56 kDa
观测分子量 50-60 kDa
GenBank蛋白编号BC013200
基因名称 LCK
Gene ID (NCBI) 3932
RRIDAB_10663101
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purification
UNIPROT IDP06239
储存缓冲液 PBS only , pH 7.3
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

LCK(Lymphocyte cell-specific protein-tyrosine kinase) is also named as LSK,p56-LCK and a nonreceptor protein-tyrosine kinase of the SRConcogene family that is involved in transduction of T-cell receptor-mediated activation.It is implicated in the control of lymphocyte growth by virtue of its overexpression in some lymphoid malignancies and its transforming activity in heterologous systems(PMID:2784463).It has 3 isoforms produced by alternative splicing.