LCK Monoclonal antibody
LCK Monoclonal Antibody for WB, IHC, IF/ICC, IF-P, FC (Intra), ELISA
Host / Isotype
Mouse / IgG1
Reactivity
human, rat, pig
Applications
WB, IHC, IF/ICC, IF-P, FC (Intra), ELISA
Conjugate
Unconjugated
CloneNo.
2D10C2
验证数据展示
经过测试的应用
Positive WB detected in | NK-92 cells, Jurkat cells, pig thymus tissue, Ramos cells, rat thymus tissue, MOLT-4 cells, MJ cells |
Positive IHC detected in | human tonsillitis tissue, human lymphoma tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Positive IF-P detected in | human tonsillitis tissue |
Positive IF/ICC detected in | HepG2 cells |
Positive FC (Intra) detected in | HepG2 cells |
推荐稀释比
Application | Dilution |
---|---|
Western Blot (WB) | WB : 1:5000-1:50000 |
Immunohistochemistry (IHC) | IHC : 1:500-1:2000 |
Immunofluorescence (IF)-P | IF-P : 1:200-1:800 |
Immunofluorescence (IF)/ICC | IF/ICC : 1:400-1:1600 |
Flow Cytometry (FC) (INTRA) | FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
发表文章中的应用
WB | See 1 publications below |
IHC | See 1 publications below |
产品信息
60162-1-Ig targets LCK in WB, IHC, IF/ICC, IF-P, FC (Intra), ELISA applications and shows reactivity with human, rat, pig samples.
Tested Applications | WB, IHC, IF/ICC, IF-P, FC (Intra), ELISA Application Description |
Cited Applications | WB, IHC |
Tested Reactivity | human, rat, pig |
Cited Reactivity | human |
Immunogen | LCK fusion protein Ag7694 种属同源性预测 |
Host / Isotype | Mouse / IgG1 |
Class | Monoclonal |
Type | Antibody |
Full Name | lymphocyte-specific protein tyrosine kinase |
Synonyms | Lymphocyte cell-specific protein-tyrosine kinase, LSK, Leukocyte C-terminal Src kinase, Lck p56, EC:2.7.10.2 |
Calculated Molecular Weight | 539 aa, 56 kDa |
Observed Molecular Weight | 50-60 kDa |
GenBank Accession Number | BC013200 |
Gene Symbol | LCK |
Gene ID (NCBI) | 3932 |
RRID | AB_10663101 |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Protein A purification |
UNIPROT ID | P06239 |
Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
实验方案
Product Specific Protocols | |
---|---|
WB protocol for LCK antibody 60162-1-Ig | Download protocol |
IHC protocol for LCK antibody 60162-1-Ig | Download protocol |
IF protocol for LCK antibody 60162-1-Ig | Download protocol |
FC protocol for LCK antibody 60162-1-Ig | Download protocol |
Standard Protocols | |
---|---|
Click here to view our Standard Protocols |
发表文章
Species | Application | Title |
---|---|---|
Int J Gen Med Identification of Biomarkers Related to Regulatory T Cell Infiltration in Oral Squamous Cell Carcinoma Based on Integrated Bioinformatics Analysis. |