LCK Monoclonal antibody

LCK Monoclonal Antibody for WB, IHC, IF/ICC, IF-P, FC (Intra), ELISA

Host / Isotype

Mouse / IgG1

Reactivity

human, rat, pig

Applications

WB, IHC, IF/ICC, IF-P, FC (Intra), ELISA

Conjugate

Unconjugated

CloneNo.

2D10C2

Cat no : 60162-1-Ig

Print datasheet

Synonyms

Lymphocyte cell-specific protein-tyrosine kinase, LSK, Leukocyte C-terminal Src kinase, Lck p56, EC:2.7.10.2



经过测试的应用

Positive WB detected inNK-92 cells, Jurkat cells, pig thymus tissue, Ramos cells, rat thymus tissue, MOLT-4 cells, MJ cells
Positive IHC detected inhuman tonsillitis tissue, human lymphoma tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF-P detected inhuman tonsillitis tissue
Positive IF/ICC detected inHepG2 cells
Positive FC (Intra) detected inHepG2 cells
Planning an IF experiment? We recommend our CoraLite® Plus 488 and CoraLite®594 conjugated versions of this antibody.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunohistochemistry (IHC)IHC : 1:500-1:2000
Immunofluorescence (IF)-PIF-P : 1:200-1:800
Immunofluorescence (IF)/ICCIF/ICC : 1:400-1:1600
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

60162-1-Ig targets LCK in WB, IHC, IF/ICC, IF-P, FC (Intra), ELISA applications and shows reactivity with human, rat, pig samples.

Tested Applications WB, IHC, IF/ICC, IF-P, FC (Intra), ELISA Application Description
Cited ApplicationsWB, IHC
Tested Reactivity human, rat, pig
Cited Reactivityhuman
Immunogen LCK fusion protein Ag7694 种属同源性预测
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Full Name lymphocyte-specific protein tyrosine kinase
Synonyms Lymphocyte cell-specific protein-tyrosine kinase, LSK, Leukocyte C-terminal Src kinase, Lck p56, EC:2.7.10.2
Calculated Molecular Weight 539 aa, 56 kDa
Observed Molecular Weight 50-60 kDa
GenBank Accession NumberBC013200
Gene Symbol LCK
Gene ID (NCBI) 3932
RRIDAB_10663101
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDP06239
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

LCK(Lymphocyte cell-specific protein-tyrosine kinase) is also named as LSK,p56-LCK and a nonreceptor protein-tyrosine kinase of the SRConcogene family that is involved in transduction of T-cell receptor-mediated activation.It is implicated in the control of lymphocyte growth by virtue of its overexpression in some lymphoid malignancies and its transforming activity in heterologous systems(PMID:2784463).It has 3 isoforms produced by alternative splicing.

实验方案

Product Specific Protocols
WB protocol for LCK antibody 60162-1-IgDownload protocol
IHC protocol for LCK antibody 60162-1-IgDownload protocol
IF protocol for LCK antibody 60162-1-IgDownload protocol
FC protocol for LCK antibody 60162-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB,IHC

Int J Gen Med

Identification of Biomarkers Related to Regulatory T Cell Infiltration in Oral Squamous Cell Carcinoma Based on Integrated Bioinformatics Analysis.

Authors - Chao Wang