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验证数据展示

  • FC experiment of A431 using CL594-60162

    FC experiment of A431 using CL594-60162

    1X10^6 A431 cells were intracellularly stained with 0.8 ug CoraLite®594 Anti-Human LCK (CL594-60162, Clone:2D10C2) (red), or 0.8 ug CoraLite®594 Mouse IgG1 Isotype Control (MOPC-21) (CL594-65124, Clone: MOPC-21) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

CoraLite®594-conjugated LCK Monoclonal antibody

LCK Monoclonal Antibody for FC (Intra)
Cat No. CL594-60162

产品说明书

CloneNo. 2D10C2

宿主/亚型

Mouse / IgG1

种属反应性

human, pig, rat

应用

FC (Intra)

别名

LCK, Lck p56, Leukocyte C-terminal Src kinase, LSK, Lymphocyte cell-specific protein-tyrosine kinase, p56-LCK, Proto-oncogene Lck, T cell-specific protein-tyrosine kinase, Tyrosine-protein kinase Lck, YT 16, YT16, 2D10C2, EC:2.7.10.2

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  CoraLite®594
规格: 

-/ -

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经过测试的应用

Positive FC (Intra) detected inA431 cells
Positive FC detected inA431 cells
For other applications, we recommend the unconjugated version of this antibody, 60162-1-Ig

推荐稀释比

应用推荐稀释比
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.80 ug per 10^6 cells in a 100 µl suspension
Flow Cytometry (FC)FC : 0.80 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL594-60162 targets LCK in FC (Intra) applications and shows reactivity with human, pig, rat samples.

经测试应用 FC (Intra) Application Description
经测试反应性 human, pig, rat
免疫原 LCK fusion protein Ag7694 种属同源性预测
宿主/亚型 Mouse / IgG1
抗体类别 Monoclonal
产品类型 Antibody
全称 lymphocyte-specific protein tyrosine kinase
别名 LCK, Lck p56, Leukocyte C-terminal Src kinase, LSK, Lymphocyte cell-specific protein-tyrosine kinase, p56-LCK, Proto-oncogene Lck, T cell-specific protein-tyrosine kinase, Tyrosine-protein kinase Lck, YT 16, YT16, 2D10C2, EC:2.7.10.2
计算分子量 539 aa, 56 kDa
观测分子量 50-60 kDa
GenBank蛋白编号BC013200
基因名称 LCK
Gene ID (NCBI) 3932
RRIDAB_3084648
偶联类型 CoraLite®594 Fluorescent Dye
最大激发/发射波长588 nm / 604 nm
形式 Liquid
纯化方式Protein G purification
UNIPROT IDP06239
储存缓冲液 PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA , pH 7.3
储存条件Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

LCK(Lymphocyte cell-specific protein-tyrosine kinase) is also named as LSK,p56-LCK and a nonreceptor protein-tyrosine kinase of the SRConcogene family that is involved in transduction of T-cell receptor-mediated activation.It is implicated in the control of lymphocyte growth by virtue of its overexpression in some lymphoid malignancies and its transforming activity in heterologous systems(PMID:2784463).It has 3 isoforms produced by alternative splicing.

实验方案

Product Specific Protocols
FC protocol for CL594 LCK antibody CL594-60162Download protocol
Standard Protocols
Click here to view our Standard Protocols