• All
  • Primary Antibodies
  • Conjugated Antibodies for IF
  • Conjugated Antibodies for FC
  • Secondary Antibodies
  • Antibody Labeling KitsNew
  • ELISA Kits
  • IHC Kits
  • Magnetic Cell Separation Kits
  • Cytokines & Growth Factors
  • Neutralizing/activating Antibodies
  • Nanobody-based Reagents
  • Accessory Products and Kits
  • Fusion Proteins
×
×
Host
0
Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
0
Compatible Species
0
Conjugate
0

CoraLite®594-conjugated LCK Monoclonal antibody

LCK Monoclonal Antibody for FC (Intra)

Host / Isotype

Mouse / IgG1

Reactivity

human, pig, rat

Applications

FC (Intra)

Conjugate

CoraLite®594 Fluorescent Dye

CloneNo.

2D10C2

Cat no : CL594-60162

Print datasheet

Synonyms

LCK, Lck p56, Leukocyte C-terminal Src kinase, LSK, Lymphocyte cell-specific protein-tyrosine kinase, p56-LCK, Proto-oncogene Lck, T cell-specific protein-tyrosine kinase, Tyrosine-protein kinase Lck, YT 16, YT16, 2D10C2, EC:2.7.10.2

验证数据展示

  • FC experiment of A431 using CL594-60162

    FC experiment of A431 using CL594-60162

    1X10^6 A431 cells were intracellularly stained with 0.8 ug CoraLite®594 Anti-Human LCK (CL594-60162, Clone:2D10C2) (red), or 0.8 ug CoraLite®594 Mouse IgG1 Isotype Control (MOPC-21) (CL594-65124, Clone: MOPC-21) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

经过测试的应用

Positive FC (Intra) detected inA431 cells
Positive FC detected inA431 cells
For other applications, we recommend the unconjugated version of this antibody, 60162-1-Ig

推荐稀释比

ApplicationDilution
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.80 ug per 10^6 cells in a 100 µl suspension
Flow Cytometry (FC)FC : 0.80 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL594-60162 targets LCK in FC (Intra) applications and shows reactivity with human, pig, rat samples.

Tested Applications FC (Intra) Application Description
Tested Reactivity human, pig, rat
Immunogen LCK fusion protein Ag7694 种属同源性预测
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Full Name lymphocyte-specific protein tyrosine kinase
Synonyms LCK, Lck p56, Leukocyte C-terminal Src kinase, LSK, Lymphocyte cell-specific protein-tyrosine kinase, p56-LCK, Proto-oncogene Lck, T cell-specific protein-tyrosine kinase, Tyrosine-protein kinase Lck, YT 16, YT16, 2D10C2, EC:2.7.10.2
Calculated Molecular Weight 539 aa, 56 kDa
Observed Molecular Weight 50-60 kDa
GenBank Accession NumberBC013200
Gene Symbol LCK
Gene ID (NCBI) 3932
Conjugate CoraLite®594 Fluorescent Dye
Excitation/Emission Maxima Wavelengths588 nm / 604 nm
Form Liquid
Purification MethodProtein G purification
UNIPROT IDP06239
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

LCK(Lymphocyte cell-specific protein-tyrosine kinase) is also named as LSK,p56-LCK and a nonreceptor protein-tyrosine kinase of the SRConcogene family that is involved in transduction of T-cell receptor-mediated activation.It is implicated in the control of lymphocyte growth by virtue of its overexpression in some lymphoid malignancies and its transforming activity in heterologous systems(PMID:2784463).It has 3 isoforms produced by alternative splicing.

实验方案

Product Specific Protocols
FC protocol for CL594 LCK antibody CL594-60162Download protocol
Standard Protocols
Click here to view our Standard Protocols