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验证数据展示

  • IF Staining of HepG2 using CL488-60162

    IF Staining of HepG2 using CL488-60162

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using CoraLite® Plus 488 LCK antibody (CL488-60162, Clone: 2D10C2 ) at dilution of 1:200, CL594-phalloidin (red).

  • FC experiment of HepG2 using CL488-60162

    FC experiment of HepG2 using CL488-60162

    1X10^6 HepG2 cells were intracellularly stained with 0.4 ug CoraLite® Plus 488 Anti-Human LCK (CL488-60162, Clone:2D10C2) (red), or 0.4 ug Control Antibody. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

CoraLite® Plus 488-conjugated LCK Monoclonal antibody

LCK Monoclonal Antibody for IF/ICC, FC (Intra)
Cat No. CL488-60162

产品说明书

CloneNo. 2D10C2

宿主/亚型

Mouse / IgG1

种属反应性

human, rat, pig

应用

IF/ICC, FC (Intra)

别名

Lymphocyte cell-specific protein-tyrosine kinase, LSK, Leukocyte C-terminal Src kinase, Lck p56, EC:2.7.10.2

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  CoraLite® Plus 488
规格: 

-/ -

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经过测试的应用

Positive IF/ICC detected inHepG2 cells
Positive FC (Intra) detected inHepG2 cells
For other applications, we recommend the unconjugated version of this antibody, 60162-1-Ig

推荐稀释比

应用推荐稀释比
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL488-60162 targets LCK in IF/ICC, FC (Intra) applications and shows reactivity with human, rat, pig samples.

经测试应用 IF/ICC, FC (Intra) Application Description
经测试反应性 human, rat, pig
免疫原 LCK fusion protein Ag7694 种属同源性预测
宿主/亚型 Mouse / IgG1
抗体类别 Monoclonal
产品类型 Antibody
全称 lymphocyte-specific protein tyrosine kinase
别名 Lymphocyte cell-specific protein-tyrosine kinase, LSK, Leukocyte C-terminal Src kinase, Lck p56, EC:2.7.10.2
计算分子量 539 aa, 56 kDa
观测分子量 50-60 kDa
GenBank蛋白编号BC013200
基因名称 LCK
Gene ID (NCBI) 3932
RRIDAB_3084140
偶联类型 CoraLite® Plus 488 Fluorescent Dye
最大激发/发射波长493 nm / 522 nm
形式 Liquid
纯化方式Protein G purification
UNIPROT IDP06239
储存缓冲液 PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
储存条件Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

LCK(Lymphocyte cell-specific protein-tyrosine kinase) is also named as LSK,p56-LCK and a nonreceptor protein-tyrosine kinase of the SRConcogene family that is involved in transduction of T-cell receptor-mediated activation.It is implicated in the control of lymphocyte growth by virtue of its overexpression in some lymphoid malignancies and its transforming activity in heterologous systems(PMID:2784463).It has 3 isoforms produced by alternative splicing.

实验方案

Product Specific Protocols
IF protocol for CL Plus 488 LCK antibody CL488-60162Download protocol
FC protocol for CL Plus 488 LCK antibody CL488-60162Download protocol
Standard Protocols
Click here to view our Standard Protocols