CoraLite® Plus 488-conjugated LCK Monoclonal antibody
LCK Monoclonal Antibody for IF/ICC, FC (Intra)
Host / Isotype
Mouse / IgG1
Reactivity
human, rat, pig
Applications
IF/ICC, FC (Intra)
Conjugate
CoraLite® Plus 488 Fluorescent Dye
CloneNo.
2D10C2
验证数据展示
经过测试的应用
Positive IF/ICC detected in | HepG2 cells |
Positive FC (Intra) detected in | HepG2 cells |
For other applications, we recommend the unconjugated version of this antibody, 60162-1-Ig
推荐稀释比
Application | Dilution |
---|---|
Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
Flow Cytometry (FC) (INTRA) | FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
产品信息
CL488-60162 targets LCK in IF/ICC, FC (Intra) applications and shows reactivity with human, rat, pig samples.
Tested Applications | IF/ICC, FC (Intra) Application Description |
Tested Reactivity | human, rat, pig |
Immunogen | LCK fusion protein Ag7694 种属同源性预测 |
Host / Isotype | Mouse / IgG1 |
Class | Monoclonal |
Type | Antibody |
Full Name | lymphocyte-specific protein tyrosine kinase |
Synonyms | Lymphocyte cell-specific protein-tyrosine kinase, LSK, Leukocyte C-terminal Src kinase, Lck p56, EC:2.7.10.2 |
Calculated Molecular Weight | 539 aa, 56 kDa |
Observed Molecular Weight | 50-60 kDa |
GenBank Accession Number | BC013200 |
Gene Symbol | LCK |
Gene ID (NCBI) | 3932 |
Conjugate | CoraLite® Plus 488 Fluorescent Dye |
Excitation/Emission Maxima Wavelengths | 493 nm / 522 nm |
Form | Liquid |
Purification Method | Protein G purification |
UNIPROT ID | P06239 |
Storage Buffer | PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3. |
Storage Conditions | Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
LCK(Lymphocyte cell-specific protein-tyrosine kinase) is also named as LSK,p56-LCK and a nonreceptor protein-tyrosine kinase of the SRConcogene family that is involved in transduction of T-cell receptor-mediated activation.It is implicated in the control of lymphocyte growth by virtue of its overexpression in some lymphoid malignancies and its transforming activity in heterologous systems(PMID:2784463).It has 3 isoforms produced by alternative splicing.
实验方案
Product Specific Protocols | |
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IF protocol for CL Plus 488 LCK antibody CL488-60162 | Download protocol |
FC protocol for CL Plus 488 LCK antibody CL488-60162 | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |