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验证数据展示

  • WB analysis using 68721-1-Ig

    WB analysis using 68721-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 68721-1-Ig (LDB1 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

LDB1 Monoclonal antibody

LDB1 Monoclonal Antibody for WB, ELISA
Cat No. 68721-1-Ig

产品说明书

CloneNo. 2F11C3

宿主/亚型

Mouse / IgG1

种属反应性

Human, mouse, rat

应用

WB, ELISA

别名

CLIM 2, CLIM2, hLdb1, LDB 1, LDB1, LIM domain binding 1, LIM domain binding protein 1, NLI, Nuclear LIM interactor

缓冲液配方:  PBS and Azide
PBS and Azide
PBS Only
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inLNCaP cells, HSC-T6 cells, NIH/3T3 cells, HeLa cells, HEK-293 cells
Planning an IHC experiment? We recommend our IHCeasy LDB1 Ready-To-Use IHC Kit. LDB1 primary antibody included.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:5000-1:50000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

68721-1-Ig targets LDB1 in WB, ELISA applications and shows reactivity with Human, mouse, rat samples.

经测试应用 WB, ELISA Application Description
经测试反应性 Human, mouse, rat
免疫原 LDB1 fusion protein Ag34563 种属同源性预测
宿主/亚型 Mouse / IgG1
抗体类别 Monoclonal
产品类型 Antibody
全称 LIM domain binding 1
别名 CLIM 2, CLIM2, hLdb1, LDB 1, LDB1, LIM domain binding 1, LIM domain binding protein 1, NLI, Nuclear LIM interactor
计算分子量 46 kDa
观测分子量 46 kDa
GenBank蛋白编号BC000482
基因名称 LDB1
Gene ID (NCBI) 8861
RRIDAB_3670413
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein G purification
UNIPROT IDQ86U70
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

LIM domain-binding protein 1 (LDB1), also known as Carboxyl-terminal LIM domain-binding protein 2 (CLIM2), binds to the LIM domain of a wide variety of LIM domain-containing transcription factors. LDB1 may regulate the transcriptional activity of LIM-containing proteins by determining specific partner interactions. It plays a role in the development of interneurons and motor neurons in cooperation with LHX3 and ISL1. LDB1 acts synergistically with LHX1/LIM1 in axis formation and activation of gene expression. It also acts with LMO2 in the regulation of red blood cell development, maintaining erythroid precursors in an immature state. LDB1 strongly interacts with the LIM2 domain of LMX1A to form homodimer. LDB1 protein is expressed in a wide range of adult tissues including brain, heart, skeletal muscle, colon, thymus, spleen, kidney, liver, small intestine, lung and peripheral blood leukocytes.

实验方案

Product Specific Protocols
WB protocol for LDB1 antibody 68721-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols