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LDHA-Specific Recombinant antibody, PBS Only

LDHA-Specific Uni-rAbTM Recombinant Antibody for IF/ICC, Indirect ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

IF/ICC, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

240885F4

Cat no : 84198-1-PBS

Print datasheet

Synonyms

LDHA, LDH A, lactate dehydrogenase A, EC:1.1.1.27, Cell proliferation-inducing gene 19 protein

验证数据展示

  • IF Staining of HepG2 using 84198-1-RR (same clone as 84198-1-PBS)

    IF Staining of HepG2 using 84198-1-RR (same clone as 84198-1-PBS)

    Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using LDHA-Specific antibody (84198-1-RR, Clone: 240885F4 ) at dilution of 1:250 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 84198-1-PBS in a different storage buffer formulation.

  • IF Staining of HepG2 using 84198-1-PBS

    IF Staining of HepG2 using 84198-1-PBS

    Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using LDHA-Specific antibody (84198-1-PBS, Clone: 240885F4 ) at dilution of 1:250 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

经过测试的应用

Positive IF/ICC detected inHepG2 cells

推荐稀释比

ApplicationDilution
Immunofluorescence (IF)/ICCIF/ICC : 1:125-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

84198-1-PBS targets LDHA-Specific in IF/ICC, Indirect ELISA applications and shows reactivity with human samples.

Tested Applications IF/ICC, Indirect ELISA Application Description
Tested Reactivity human
Immunogen Peptide 种属同源性预测
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Full Name lactate dehydrogenase A
Synonyms LDHA, LDH A, lactate dehydrogenase A, EC:1.1.1.27, Cell proliferation-inducing gene 19 protein
Calculated Molecular Weight 37 kDa
GenBank Accession NumberNM_005566
Gene Symbol LDHA
Gene ID (NCBI) 3939
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purfication
UNIPROT IDP00338
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

LDHA, also named as LDH-M and NY-REN-59, is an enzyme which catalyzes the inter-conversion of pyruvate and L-lactate with concomitant inter-conversion of NADH and NAD+. LDHA is found in most somatic tissues, though predominantly in muscle tissue and tumours, and belongs to the lactate dehydrogenase family. It has long been known that many human cancers have higher LDHA levels compared to normal tissues. It has also been shown that LDHA plays an important role in the development, invasion and metastasis of malignancies.

实验方案

Product Specific Protocols
IF protocol for LDHA-Specific antibody 84198-1-PBSDownload protocol
Standard Protocols
Click here to view our Standard Protocols