• All
  • Primary Antibodies
  • Conjugated Antibodies for IF
  • Conjugated Antibodies for FC
  • Secondary Antibodies
  • Antibody Labeling KitsNew
  • ELISA Kits
  • IHC Kits
  • Magnetic Cell Separation Kits
  • Cytokines & Growth Factors
  • Neutralizing/activating Antibodies
  • Nanobody-based Reagents
  • Accessory Products and Kits
  • Fusion Proteins
×
×
Host
0
Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
0
Compatible Species
0
Conjugate
0

验证数据展示

  • IF Staining of HeLa using 66425-1-Ig (same clone as 66425-1-PBS)

    IF Staining of HeLa using 66425-1-Ig (same clone as 66425-1-PBS)

    Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using 66425-1-Ig (LDHB antibody), at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 66425-1-PBS in a different storage buffer formulation.

  • IHC staining of mouse heart using 66425-1-Ig (same clone as 66425-1-PBS)

    IHC staining of mouse heart using 66425-1-Ig (same clone as 66425-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse heart tissue slide using 66425-1-Ig (LDHB antibody) at dilution of 1:32000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66425-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with LDHB antibody included
  • WB analysis using 66425-1-Ig (same clone as 66425-1-PBS)

    WB analysis using 66425-1-Ig (same clone as 66425-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 66425-1-Ig (LDHB antibody) at dilution of 1:50000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated Beta Actin Monoclonal antibody (HRP-66009) as loading control. This data was developed using the same antibody clone with 66425-1-PBS in a different storage buffer formulation.

LDHB Monoclonal antibody, PBS Only

LDHB Monoclonal Antibody for WB, IHC, IF/ICC, ELISA
Cat No. 66425-1-PBS

产品说明书

CloneNo. 5E9G1

宿主/亚型

Mouse / IgG1

种属反应性

human, rat, mouse, pig

应用

WB, IHC, IF/ICC, ELISA

别名

lactate dehydrogenase B, LDH B, LDH H, LDH heart subunit, LDHB, LDH-B, TRG 5

缓冲液配方:  PBS Only
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格: 

-/ -

点击咨询售前客服

点击咨询售后客服

Planning an IHC experiment? We recommend our IHCeasy LDHB Ready-To-Use IHC Kit. LDHB primary antibody included.

产品信息

66425-1-PBS targets LDHB in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, rat, mouse, pig samples.

经测试应用 WB, IHC, IF/ICC, ELISA Application Description
经测试反应性 human, rat, mouse, pig
免疫原 LDHB fusion protein Ag6605 种属同源性预测
宿主/亚型 Mouse / IgG1
抗体类别 Monoclonal
产品类型 Antibody
全称 lactate dehydrogenase B
别名 lactate dehydrogenase B, LDH B, LDH H, LDH heart subunit, LDHB, LDH-B, TRG 5
计算分子量 36 kDa
观测分子量 35 kDa
GenBank蛋白编号BC002362
基因名称 LDHB
Gene ID (NCBI) 3945
RRIDAB_2881796
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purification
UNIPROT IDP07195
储存缓冲液 PBS Only
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

LDHB, also named as LDH-H and NY-REN-46, belongs to the LDH/MDH superfamily. And LDH family. It is an enzyme which catalyzes the reversible conversion of lactate and pyruvate, and NAD and NADH, in the glycolytic pathway.It is glycolytic enzymes and it is positively regulated by mTOR.