LDHB Monoclonal antibody
LDHB Monoclonal Antibody for WB, IHC, IF/ICC, ELISA
Host / Isotype
Mouse / IgG1
Reactivity
human, rat, mouse, pig
Applications
WB, IHC, IF/ICC, ELISA and More (2)
Conjugate
Unconjugated
CloneNo.
5E9G1
验证数据展示
经过测试的应用
Positive WB detected in | HEK-293 cells, PC-12 cells, Neuro-2a cells, pig brain tissue, rat brain tissue, mouse brain tissue, rabbit brain tissue, mouse cerebellum tissue, rat cerebellum tissue |
Positive IHC detected in | mouse heart tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Positive IF/ICC detected in | HeLa cells |
推荐稀释比
Application | Dilution |
---|---|
Western Blot (WB) | WB : 1:20000-1:100000 |
Immunohistochemistry (IHC) | IHC : 1:16000-1:64000 |
Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
产品信息
66425-1-Ig targets LDHB in WB, IHC, IF/ICC, CoIP, ELISA applications and shows reactivity with human, rat, mouse, pig samples.
Tested Applications | WB, IHC, IF/ICC, ELISA Application Description |
Cited Applications | WB, IF, CoIP |
Tested Reactivity | human, rat, mouse, pig |
Cited Reactivity | human, mouse, rat |
Immunogen | LDHB fusion protein Ag6605 种属同源性预测 |
Host / Isotype | Mouse / IgG1 |
Class | Monoclonal |
Type | Antibody |
Full Name | lactate dehydrogenase B |
Synonyms | lactate dehydrogenase B, LDH B, LDH H, LDH heart subunit, LDHB, LDH-B, TRG 5 |
Calculated Molecular Weight | 36 kDa |
Observed Molecular Weight | 35 kDa |
GenBank Accession Number | BC002362 |
Gene Symbol | LDHB |
Gene ID (NCBI) | 3945 |
RRID | AB_2881796 |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Protein A purification |
UNIPROT ID | P07195 |
Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
实验方案
Product Specific Protocols | |
---|---|
WB protocol for LDHB antibody 66425-1-Ig | Download protocol |
IHC protocol for LDHB antibody 66425-1-Ig | Download protocol |
IF protocol for LDHB antibody 66425-1-Ig | Download protocol |
Standard Protocols | |
---|---|
Click here to view our Standard Protocols |
发表文章
Species | Application | Title |
---|---|---|
Cell Metab Augmentation of scleral glycolysis promotes myopia through histone lactylation | ||
Acta Pharm Sin B Co-delivery of photosensitizer and diclofenac through sequentially responsive bilirubin nanocarriers for combating hypoxic tumors. | ||
Life Sci Aerobic exercise reduced the amount of CHRONO bound to BMAL1 and ameliorated glucose metabolic dysfunction in skeletal muscle of high-fat diet-fed mice | ||
Cell Death Dis PDK4 promotes vascular calcification by interfering with autophagic activity and metabolic reprogramming. | ||
Life (Basel) Skeletal-Muscle-Specific Overexpression of Chrono Leads to Disruption of Glucose Metabolism and Exercise Capacity | ||
PeerJ Creatine kinase mitochondrial 2 promotes the growth and progression of colorectal cancer via enhancing Warburg effect through lactate dehydrogenase B |