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CoraLite®594-conjugated LDHB Monoclonal antibody

LDHB Monoclonal Antibody for IF/ICC

Host / Isotype

Mouse / IgG1

Reactivity

human, rat, mouse

Applications

IF/ICC

Conjugate

CoraLite®594 Fluorescent Dye

CloneNo.

5E9G1

Cat no : CL594-66425

Print datasheet

Synonyms

lactate dehydrogenase B, LDH B, LDH H, LDH heart subunit, LDHB, TRG 5

验证数据展示

  • IF Staining of HeLa using CL594-66425

    IF Staining of HeLa using CL594-66425

    Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using CoraLite®594 LDHB antibody (CL594-66425, Clone: 5E9G1 ) at dilution of 1:200.

经过测试的应用

Positive IF/ICC detected inHeLa cells
Planning an IHC experiment? We recommend our IHCeasy LDHB Ready-To-Use IHC Kit. LDHB primary antibody included.
For other applications, we recommend the unconjugated version of this antibody, 66425-1-Ig

推荐稀释比

ApplicationDilution
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL594-66425 targets LDHB in IF/ICC applications and shows reactivity with human, rat, mouse samples.

Tested Applications IF/ICC Application Description
Tested Reactivity human, rat, mouse
Immunogen LDHB fusion protein Ag6605 种属同源性预测
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Full Name lactate dehydrogenase B
Synonyms lactate dehydrogenase B, LDH B, LDH H, LDH heart subunit, LDHB, TRG 5
Calculated Molecular Weight 36 kDa
Observed Molecular Weight 35 kDa
GenBank Accession NumberBC002362
Gene Symbol LDHB
Gene ID (NCBI) 3945
RRIDAB_2919989
Conjugate CoraLite®594 Fluorescent Dye
Excitation/Emission Maxima Wavelengths588 nm / 604 nm
Form Liquid
Purification MethodProtein G purification
UNIPROT IDP07195
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

LDHB, also named as LDH-H and NY-REN-46, belongs to the LDH/MDH superfamily. And LDH family. It is an enzyme which catalyzes the reversible conversion of lactate and pyruvate, and NAD and NADH, in the glycolytic pathway.It is glycolytic enzymes and it is positively regulated by mTOR.

实验方案

Product Specific Protocols
IF protocol for CL594 LDHB antibody CL594-66425Download protocol
Standard Protocols
Click here to view our Standard Protocols