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Host
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Species Reactivity
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Species Reactivity
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Conjugate
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Conjugate
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Compatible Species
0
Conjugate
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验证数据展示

  • WB analysis using 81963-1-RR (same clone as 81963-1-PBS)

    WB analysis using 81963-1-RR (same clone as 81963-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 81963-1-RR (LDHB antibody) at dilution of 1:100000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 81963-1-PBS in a different storage buffer formulation.

  • IP experiment of HeLa using 81963-1-RR (same clone as 81963-1-PBS)

    IP experiment of HeLa using 81963-1-RR (same clone as 81963-1-PBS)

    IP result of anti-LDHB (IP:81963-1-RR, 4ug; Detection:81963-1-RR 1:50000) with HeLa cells lysate 2000 ug. This data was developed using the same antibody clone with 81963-1-PBS in a different storage buffer formulation.

  • IF Staining of HeLa using 81963-1-RR (same clone as 81963-1-PBS)

    IF Staining of HeLa using 81963-1-RR (same clone as 81963-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using LDHB antibody (81963-1-RR, Clone: 1M20 ) at dilution of 1:1000 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red). This data was developed using the same antibody clone with 81963-1-PBS in a different storage buffer formulation.

  • FC experiment of HeLa using 81963-1-RR (same clone as 81963-1-PBS)

    FC experiment of HeLa using 81963-1-RR (same clone as 81963-1-PBS)

    1X10^6 HeLa cells were intracellularly stained with 0.4 ug Anti-Human LDHB (81963-1-RR, Clone:1M20) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Isotype Control. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 81963-1-PBS in a different storage buffer formulation.

LDHB Recombinant antibody, PBS Only

LDHB Uni-rAbTM Recombinant Antibody for WB, IF/ICC, FC (Intra), IP, Indirect ELISA
Cat No. 81963-1-PBS

产品说明书

CloneNo. 1M20

宿主/亚型

Rabbit / IgG

种属反应性

Human, mouse, rat

应用

WB, IF/ICC, FC (Intra), IP, Indirect ELISA

别名

lactate dehydrogenase B, LDH H, LDH B, LDH, EC:1.1.1.27

缓冲液配方:  PBS Only
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格: 

-/ -

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Planning an IHC experiment? We recommend our IHCeasy LDHB Ready-To-Use IHC Kit. LDHB primary antibody included.

产品信息

81963-1-PBS targets LDHB in WB, IF/ICC, FC (Intra), IP, Indirect ELISA applications and shows reactivity with Human, mouse, rat samples.

经测试应用 WB, IF/ICC, FC (Intra), IP, Indirect ELISA Application Description
经测试反应性 Human, mouse, rat
免疫原 LDHB fusion protein Ag6605 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 lactate dehydrogenase B
别名 lactate dehydrogenase B, LDH H, LDH B, LDH, EC:1.1.1.27
计算分子量 36 kDa
观测分子量 35 kDa
GenBank蛋白编号BC002362
基因名称 LDHB
Gene ID (NCBI) 3945
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purification
UNIPROT IDP07195
储存缓冲液 PBS Only
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

LDHB, also named as LDH-H and NY-REN-46, belongs to the LDH/MDH superfamily. And LDH family. It is an enzyme which catalyzes the reversible conversion of pyruvate and NADH to lactate and NAD+ in the glycolytic pathway. LDH comprises LDHA and LDHB, two subunits that are encoded by independent genes. In muscle or liver, most of the LDH is composed of four LDHA subunits, and preferably catalyzes the reduction of pyruvate to lactate. In heart and brain, LDH is mainly composed of four LDHB subunits, and predominantly catalyzes the oxidation of lactate to pyruvate. In other tissues, LDH is composed of both LDHA and LDHB. (PMID: 26269128, 31804482)