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LDHB Recombinant antibody

LDHB Uni-rAbTM Recombinant Antibody for WB, IF/ICC, FC (Intra), IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

Human, mouse, rat

Applications

WB, IF/ICC, FC (Intra), IP, ELISA

Conjugate

Unconjugated

CloneNo.

1M20

Cat no : 81963-1-RR

Print datasheet

Synonyms

lactate dehydrogenase B, LDH H, LDH B, LDH, EC:1.1.1.27

验证数据展示

  • WB analysis using 81963-1-RR

    WB analysis using 81963-1-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 81963-1-RR (LDHB antibody) at dilution of 1:100000 incubated at room temperature for 1.5 hours.

  • IP experiment of HeLa using 81963-1-RR

    IP experiment of HeLa using 81963-1-RR

    IP result of anti-LDHB (IP:81963-1-RR, 4ug; Detection:81963-1-RR 1:50000) with HeLa cells lysate 2000 ug.

  • IF Staining of HeLa using 81963-1-RR

    IF Staining of HeLa using 81963-1-RR

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using LDHB antibody (81963-1-RR, Clone: 1M20 ) at dilution of 1:1000 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red).

  • FC experiment of HeLa using 81963-1-RR

    FC experiment of HeLa using 81963-1-RR

    1X10^6 HeLa cells were intracellularly stained with 0.4 ug Anti-Human LDHB (81963-1-RR, Clone:1M20) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Isotype Control. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

经过测试的应用

Positive WB detected inHeLa cells, HEK-293 cells, DU 145 cells, rat heart tissue, mouse brain tissue, rat brain tissue
Positive IP detected inHeLa cells
Positive IF/ICC detected inHeLa cells
Positive FC (Intra) detected inHeLa cells
Planning an IHC experiment? We recommend our IHCeasy LDHB Ready-To-Use IHC Kit. LDHB primary antibody included.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:20000-1:100000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunofluorescence (IF)/ICCIF/ICC : 1:500-1:2000
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

81963-1-RR targets LDHB in WB, IF/ICC, FC (Intra), IP, ELISA applications and shows reactivity with Human, mouse, rat samples.

Tested Applications WB, IF/ICC, FC (Intra), IP, ELISA Application Description
Tested Reactivity Human, mouse, rat
Immunogen LDHB fusion protein Ag6605 种属同源性预测
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Full Name lactate dehydrogenase B
Synonyms lactate dehydrogenase B, LDH H, LDH B, LDH, EC:1.1.1.27
Calculated Molecular Weight 36 kDa
Observed Molecular Weight 35 kDa
GenBank Accession NumberBC002362
Gene Symbol LDHB
Gene ID (NCBI) 3945
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDP07195
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

LDHB, also named as LDH-H and NY-REN-46, belongs to the LDH/MDH superfamily. And LDH family. It is an enzyme which catalyzes the reversible conversion of pyruvate and NADH to lactate and NAD+ in the glycolytic pathway. LDH comprises LDHA and LDHB, two subunits that are encoded by independent genes. In muscle or liver, most of the LDH is composed of four LDHA subunits, and preferably catalyzes the reduction of pyruvate to lactate. In heart and brain, LDH is mainly composed of four LDHB subunits, and predominantly catalyzes the oxidation of lactate to pyruvate. In other tissues, LDH is composed of both LDHA and LDHB. (PMID: 26269128, 31804482)

实验方案

Product Specific Protocols
WB protocol for LDHB antibody 81963-1-RRDownload protocol
IF protocol for LDHB antibody 81963-1-RRDownload protocol
IP protocol for LDHB antibody 81963-1-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols