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LEPR Recombinant antibody

LEPR Uni-rAbTM Recombinant Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, ELISA

Conjugate

Unconjugated

CloneNo.

241692G9

Cat no : 84464-4-RR

Print datasheet

Synonyms

LEP-R, LEP R, HuB219, DB, CD295

验证数据展示

  • WB analysis using 84464-4-RR

    WB analysis using 84464-4-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 84464-4-RR (LEPR antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

经过测试的应用

Positive WB detected inK-562 cells, HeLa cells, HepG2 cells, L02 cells, mouse brain tissue

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

84464-4-RR targets LEPR in WB, ELISA applications and shows reactivity with human, mouse samples.

Tested Applications WB, ELISA Application Description
Tested Reactivity human, mouse
Immunogen Recombinant Protein 种属同源性预测
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Full Name leptin receptor
Synonyms LEP-R, LEP R, HuB219, DB, CD295
Calculated Molecular Weight 132 kDa
Observed Molecular Weight120 kDa
GenBank Accession NumberNM_002303
Gene Symbol LEPR
Gene ID (NCBI) 3953
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purfication
UNIPROT IDP48357
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

The hormone leptin, secreted predominantly from adipose tissue, plays a crucial role in the regulation of numerous neuroendocrine functions, from energy homeostasis to reproduction (PMID: 22249808). Leptin acts through the leptin receptor (LEPR, also known as OB-R), a single-transmembrane-domain receptor of the cytokine receptor family. On ligand binding, LEPR mediates signaling through JAK2/STAT3 (PMID: 9102398). LEPR plays an important role in the regulation of glucose homeostasis via feeding and adiposity. Alternatively spliced transcript variants encoding different isoforms of LEPR have been described.

实验方案

Product Specific Protocols
WB protocol for LEPR antibody 84464-4-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols