验证数据展示
经过测试的应用
| Positive WB detected in | K-562 cells, HeLa cells, HepG2 cells, L02 cells, mouse brain tissue |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
84464-4-RR targets LEPR in WB, ELISA applications and shows reactivity with human, mouse samples.
| 经测试应用 | WB, ELISA Application Description |
| 经测试反应性 | human, mouse |
| 免疫原 | Recombinant Protein 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Recombinant |
| 产品类型 | Antibody |
| 全称 | leptin receptor |
| 别名 | LEP-R, LEP R, HuB219, DB, CD295 |
| 计算分子量 | 132 kDa |
| 观测分子量 | 120 kDa |
| GenBank蛋白编号 | NM_002303 |
| 基因名称 | LEPR |
| Gene ID (NCBI) | 3953 |
| RRID | AB_3671986 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Protein A purfication |
| UNIPROT ID | P48357 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
The hormone leptin, secreted predominantly from adipose tissue, plays a crucial role in the regulation of numerous neuroendocrine functions, from energy homeostasis to reproduction (PMID: 22249808). Leptin acts through the leptin receptor (LEPR, also known as OB-R), a single-transmembrane-domain receptor of the cytokine receptor family. On ligand binding, LEPR mediates signaling through JAK2/STAT3 (PMID: 9102398). LEPR plays an important role in the regulation of glucose homeostasis via feeding and adiposity. Alternatively spliced transcript variants encoding different isoforms of LEPR have been described.
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for LEPR antibody 84464-4-RR | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |