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验证数据展示

  • WB analysis of HeLa using 68299-1-Ig

    WB analysis of HeLa using 68299-1-Ig

    HeLa cells were subjected to SDS PAGE followed by western blot with 68299-1-Ig (LEPREL2 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • WB analysis of rat testis using 68299-1-Ig

    WB analysis of rat testis using 68299-1-Ig

    rat testis tissue were subjected to SDS PAGE followed by western blot with 68299-1-Ig (LEPREL2 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • IHC staining of mouse ovary using 68299-1-Ig

    IHC staining of mouse ovary using 68299-1-Ig

    Immunohistochemical analysis of paraffin-embedded mouse ovary tissue slide using 68299-1-Ig (LEPREL2 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

LEPREL2 Monoclonal antibody

LEPREL2 Monoclonal Antibody for WB, IHC, ELISA
Cat No. 68299-1-Ig

产品说明书

CloneNo. 1F6F3

宿主/亚型

Mouse / IgG1

种属反应性

Human, Rat, Mouse

应用

WB, IHC, ELISA

别名

GRCB, HSU47926, leprecan like 2, Leprecan like protein 2, LEPREL2, P3H3, Prolyl 3 hydroxylase 3, Protein B

缓冲液配方:  PBS and Azide
PBS and Azide
PBS Only
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inrat testis tissue, HeLa cells
Positive IHC detected inmouse ovary tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:5000-1:50000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

68299-1-Ig targets LEPREL2 in WB, IHC, ELISA applications and shows reactivity with Human, Rat, Mouse samples.

经测试应用 WB, IHC, ELISA Application Description
经测试反应性 Human, Rat, Mouse
免疫原 LEPREL2 fusion protein Ag8850 种属同源性预测
宿主/亚型 Mouse / IgG1
抗体类别 Monoclonal
产品类型 Antibody
全称 leprecan-like 2
别名 GRCB, HSU47926, leprecan like 2, Leprecan like protein 2, LEPREL2, P3H3, Prolyl 3 hydroxylase 3, Protein B
计算分子量 736 aa, 82 kDa
观测分子量82 kDa
GenBank蛋白编号BC017217
基因名称 LEPREL2
Gene ID (NCBI) 10536
RRIDAB_2935379
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein G purification
UNIPROT IDQ8IVL6
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

P3H3 (prolyl 3-hydroxylase 3, also known as LEPREL2) is a member of the leprecan family of proteins, which also include P3H1, P3H2, CRTAP and SC56. Collagen prolyl hydroxylases are required for proper collagen biosynthesis, folding, and assembly. P3H3 is thought to reside in the endoplasmic reticulum and has prolyl 3-hydroxylase activity catalyzing the post-translational formation of 3-hydroxyproline in -Xaa-Pro-Gly-sequences in collagens, especially types IV and V.

实验方案

Product Specific Protocols
WB protocol for LEPREL2 antibody 68299-1-IgDownload protocol
IHC protocol for LEPREL2 antibody 68299-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols